776 SUMMARY OF CURRENT RESEARCHES RELATING TO 



since it was first published as Elementary Microscopy. The second 

 edition is called Practical Microscopy, an Introduction to Microscopical 

 Methods. Much new matter has been incorporated, the scope of the 

 book has been considerably enlarged, it has been nearly doubled in size, 

 and much has been re-written, notably chapters iii. and vi., dealing with 

 the choice of a Microscope, and with the practical optics of the Micro- 

 scope. An entirely new chapter on Photomicrography has been added, 

 and that on Microscopical Technique has been materially amplified. 



B. Technique.* 

 CI) Collecting' Objects, including- Culture Processes. 



Study of Tubercle bacilli.f — C. Siebert, from observations of two 

 strains of tubercle bacillus, found that in glycerin broth the growth is 

 associated with the production of acid ; there is no difference in the 

 acid formation of human tubercle and bovine tubercle. The amount of 

 growth of tubercle bacilli in broth is greater if the acid formed is 

 neutralised with soda, or if a piece of marble is added to the medium. 



Dextrose and Lactose for Detecting the Colon Bacillus.:}: — 

 W. R. Stokes and H. W. Stouer consider that for the detection of the 

 colon bacillus, dextrose, lactose, and saccharose broths should all be 

 used, and that the organism should be plated out in pure culture. If one 

 sugar must be used to identify sub-cultures, it should be lactose, and not 

 dextrose, because there are many other types of organism that ferment 

 dextrose exactly like B. coll. The authors consider that any organism 

 producing 18-80 p.c. of gas in dextrose, lactose, and saccharose 

 broths might be regarded as B. coli if it also showed the usual cultural 

 characters. 



Normal Cerebrospinal Fluid as Nutrient Medium for Patho- 

 genic Bacteria. § — L. Yincenzi finds that Biplococcus pneumoniae, 

 Gonococcus, Streptococcus erysipelatis, and the bacilli of plague, diph- 

 theria, and tubercle do not grow in normal cerebrospinal fluid. Staphy- 

 lococcus aureus and anthrax grow badly, while B. typhi, B. coli, B. para- 

 typhi A and B, V. cholerse, B. dysentericus Shiga, and M. melitensis grow 

 fairly well but not luxuriantly. 



Shaker (Kinotherm), for Use at a Desired Temperature. || — 

 P.Uhlenhuth has devised this apparatus (fig. 123) for shaking any sub- 

 stance such as bacterial extracts, etc., in a vessel. The motion is imparted 



* This subdivision contains (1) Collecting Objects, including Culture Pro- 

 cesses ; (2) Preparing Objects; (3) Cutting, including Imbedding and Microtomes ; 

 (4) Staining and Injecting ; (5) Mounting, including slides, preservative fluids, etc. ; 

 (6) Miscellaneous. 



t Centralbl. Bakt., lte Abt. Orig., li. (1909) p 305. 



t Tom. cit., p. 459. § Op cit., Iii. (1909) pp. 154-6. 



§ Zeitschr. f. Immunitat. forsch. u. exper. Therapie, ii. (1909) No. 3, through 

 Centralbl. Bakt., lte Abt. Ref., xliv. (1909) pp. 629-31 (1 fig.). 



