ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 783 



solutions are mixed in the proportion of 10 to 1 ; after a good shaking 

 the solution is ready for use. On removal from the stain the sections 

 are washed in water and mounted in glycerin, glycerin jelly, or balsam. 



Modification of Nissl's Method.* — Rodenwaldt describes a simplified 

 Nissl method and its application to Beriberi. A solution of Azur ii. in 

 the proportion of 1 grm. to 750 c.cm. of distilled water is made, and 

 to 10 c.cm. of this solution i drops of a saturated solution of potassium- 

 carbonate are added immediately before use. 



Van Gieson and Romanowsky Stains for Detection of Coccidia.t, 

 P. B. Hadlev recommends Van <4ieson"s stain for rapidly detecting 

 coccidia in the intestines or ca3ca. It is made as follows : To 10 c.cm. 

 of distilled water add 2 drops of saturated 95 p.c. alcoholic solution of 

 rose-anilin-violet and 10 drops of a 50 p.c. solution of methylen-blue. 

 The smears, made in the usual way, are fixed in alcohol. The stain is 

 heated to vaporisation, and then the slide is washed in water, mopped 

 up with blotting paper and dried in air. 



The Romanowsky stain was used for demonstrating the finer nuclear 

 structure of different stages of Coccidium cunicuU. 



Demonstrating' the Intracellular Network of Nerve-cells.J — 

 F. Marcora places the pieces for 7 to fS hours in a mixture consisting of 

 arsenious acid 0*75 p.c, 40 parts ; formol. 10 parts. After this they 

 are immersed for 12 hours in 2 p.c. silver nitrate solution. The 

 developer consists of hydrochinon 20, sodium sulphite 5, formol 50, 

 distilled water 1000. After this the pieces are cleared up and imbedded 

 in paraffin. The sections, which should be 10-15 /m thick, are then 

 passed through downgraded alcohols to water, and afterwards treated with 

 the following mixture, which is composed of two solutions. Solution A 

 contains sodium hyposulphite 30, ammonium rhodanate 30, distilled 

 water 1000. Solution B : gold chloride 1, distilled water 100. The 

 sections are next washed in distilled water and then bleached by the fol- 

 lowing method. For 5 to 10 minutes the sections are immersed in the 

 following mixture : potassium permanganate 0*5, sulphuric acid 1, dis- 

 tilled water 1000 ; and then passed rapidly through a 1 p.c. solution of 

 oxalic acid, after which they are frequently washed in distilled water. 

 This is followed by Xissl's procedure, viz. staining with an aqueous 

 solution of magenta-red warmed to vaporisation : then 95 p.c. alcohol and 

 differentiating in oil of cloves. Dehydration ; xylol ; balsam. By this 

 method not only is the intracellular network well shown, but it also 

 demonstrates that Nissl's bodies lie in the interspaces of the network. 



Staining- Treponema pallidum.§ — F. L. deYerteuil fixed smears in 

 absolute alcohol and then stained them with 10 p.c. silver nitrate for an 

 hour at about 100~ F. and afterwards reduced with 5 p.c. pyrogallic acid 

 for 10 minutes. 



* Monats-schrift. f. Psych, u. Neurol. April 1908. See also Zeitschr. wiss. 

 Mikrosk., xxv. (1909) pp. 332. 



t Centralbl. Bakt., lte Abt. Grig., lii. (1909) pp. 147-50. 

 X Anat. Anzeig., xxxv. (1909) pp. 65-9(1 fig.).' 

 § Lancet, 1909, ii. pp. 884-5. 



