ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 531 



the operating theatre to the laboratory, during which time the specimen 

 lies in acetone, the author has found that on an average the whole 

 time occupied, from the removal of the tissue till the diagnosis is given 

 to the surgeon, is 7 minutes. 



Combined Imbedding Method.* — Herzog Gandolfi hardens and 

 dehydrates the object in upgraded alcohols, and then transfers to 

 a mixture of equal parts of toluol and absolute alcohol for a day, and 

 afterwards to a solution of celloidin in the foregoing fluids and having 

 the consistence of cedar wood or olive oil. According to its nature the 

 object requires from 3 to 7 days saturation. When sufficiently saturated 

 the object is transferred to chloroform, in which are placed a few drops 

 of ether and some bits of paraffin, and incubated at about 56° for some 

 15 minutes, and then transferred to pure paraffin for 30 minutes or so, 

 after which it is imbedded and sectioned in the usual way. 



Hoyer, H. — Eine neue Vorrichtung zu Injektionen. 



[An apparatus intended for injecting small animals.] 



Zeitschr. wiss. Mikrosk., xxv. (1909) pp. 412-20 (3 figs.). 

 Ssobelew, L. W. — Zur Celloidintechnik. 



[Gives the author's precedure in minute detail for dealing with a celloidin 

 section.] Tom. cit., pp. 410-12. 



(4) Staining- and Injecting:. 



Differentiating Ergastoplasm and Mitochondria in the Human 

 Submaxillary Gland. t — 0. Regaud and J. Mawas find from their 

 examinations that the mitochondria and ergastoplasm differ in shape, 

 situation, and histochemical reactions. They deduce their conclusions 

 from a comparative study of four kinds of preparation, viz. : (1) 

 fixation with fluid containing 5 p.c. acetic acid : (2) fixation with 

 formalin and chromic acid or a chromate. The tissues thus fixed were 

 stained with (1) ha3inaluni and (2) iron-hrematoxylin. 



New Method for Staining Spirochseta pallida in Tissues. $— 

 J. Yamanioto has modified the original procedure in the following 

 way. The tissue to be investigated may be preserved in various 

 solutions, and is then cat into small pieces 10 mm. long and 5 mm. in 

 thickness and breadth. These are washed free from the fixing 

 medium by rinsing in water for 2-1 hours, and finally in distilled 

 water for one hour. Each piece is then put into 10 c.cm. of a 5 p.c. 

 solution of silver nitrate in brown-coloured bottles and kept at 37° C. 

 for 48 hours. At the end of this time they are placed in a reducing 

 -solution containing 2 p.c. of pyrogallic acid and 1 p.c. of tannic acid 

 in distilled water in similar brown bottles, in which they are kept for 

 24 hours at 37° 0. It is, however, necessary to change this solution 

 after the first half-hour, since it is rendered turbid by the reduction. 

 After this the pieces of tissue are placed in water for an hour, and 

 then washed in alcohol of increasing strength until decolorised ; 

 they are then imbedded in paraffin or celloidin, the latter giving better 



* Zeitschr. wiss. Mikrosk., xxv. (1909) pp. 421-2. 

 t C.R. Soc. Biol. Paris, lxvi. (1909) pp. 461-3. 



X Centralbl. allgem. Pathol, u. pathol. Anat., Feb. 29, 1909. See also Lancet, 

 (1909) i. p. 933. 



2 n 2 



