ZOOLOGY AND BOTANY, MICKOSCOPY, ETC. 385 



fresh ammonia-alcohol for 1-2 days. On removal they were washed for 

 5-10 minutes in distilled water, and then impregnated with 1 p.c. silver 

 nitrate (3-6 days at 35°-37° C). On removal the pieces were dried 

 with blotting paper, and then developed in diffused light in pyrogallol 

 solution (pyrogallol 2, formalin 5, distilled water 100). This was followed 

 by alcohol, chloroform, imbedding in paraffin, sectioning. The sections 

 were placed in 1 p.c. gold chloride solution for 5-10 minutes, and then 

 in 5 p.c. sodium hyposulphite for 5-10 minutes, after which they were 

 mounted. If the sections be too thick it is advisable to omit the gold 

 stage. Pieces thus treated are free from precipitate. 



Differential Staining of Typhoid Bacilli in Sections.* — H. Bonhoff 

 recommends a modification of Pick and Jacobsohn's method of demon- 

 strating gonococci in tissues for the differential staining of Bacillus 

 typhosus in sections. Instead of 8, he uses 4 drops of a saturated 

 alcoholic solution of methylen-blue, and adds these to 15 drops of Ziehl's 

 solution, and 10 c.cm. of distilled water. The stain is first allowed to 

 act for 2 minutes cold, and is then gently warmed. 1 p.c. acetic acid is 

 used for differentiating. After washing, the section is dehydrated in 

 anilin-xylol (equal parts). The typhoid bacilli are deep blue on a red 

 background. 



Spore Staining.f — E. Thesing mordants the films with hot 1 p.c. 

 platinum chloride solution. After washing and drying, the film is hot 

 stained, and then thoroughly washed with 33 p.c. alcohol. The film is 

 again dried and contrast-stained in the cold for 3 minutes. 



New Method of Spore Staining.^ — Scagliosi recommends that the 

 material should be fixed with van Gehuchten's or Hermann's fluid. 

 After staining with carbol-fuchsin, wash in water or dilute sulphuric 

 acid, and contrast-stain with methylen-blue. 



Method of Staining Sensory Nerve Sheaths.§ — A. Ruffini describes 

 a method for staining the subsidiary sheath of sensory nerves. (1) Small 

 pieces of skin or muscle are left for half an hour or more in a solution 

 composed of 20 p.c. formic acid 66 parts, and hot saturated aqueous 

 solution of sublimate 34 parts. This mixture must be prepared some 

 time in advance. (2) The pieces are washed quickly in running water. 

 (3) They are placed for 20-40 minutes in 1 p.c. solution of gold 

 chloride. (4) They are mopped up with blotting paper, and placed in 

 2 p.c. solution of formic acid, and kept in the dark for 12-15 hours. 

 (5) The vessel is then exposed to sunlight for 6-8 hours. (6) The 

 pieces are dried carefully and placed in glycerin. (7) After 8-10 days 

 they are teased out and mounted in glycerin. 



New Method for Staining Glycogen. || — A. Fischer describes the 

 following method for staining glycogen, which was tested on the liver 

 of the pig and mouse. Fixation in alcohol : the paraffin sections are 



* Archiv Hygien, 1., No. 3. See also Zeitsch. angew. Mikr., x. (1905) p. 301. 

 + Loo. cit. See also Zeitschr. angew. Mikr., x. (1905) p. 306. 

 % Riforma Med., 1904, No. 49. See also Centralbl. Bakt, l te Abt. Ref., xxxvi. 

 (1905) pp. 2K3. § Zeitschr. wiss. Zool., lxxix. (1905) p. 151 (2 pis.). 



|| Anat. \nzeig., xxvi. (1905) pp. 399-400. 



June 21st, 1905 2 d 



