386 SUMMARY OF CURRENT RESEARCHES RELATING TO 



placed in alcohol and passed straight away to a 10 p.c. aqueous solution 

 of tannin for 10-15 minutes. The sections are washed in 1 p.c. solution 

 of potassium bichromate and then placed for 10-15 minutes in 10 p.c. 

 potassium bichromate for fixation. The glycogen is by this time almost 

 insoluble, and will stand washing with water and staining with aqueous 

 solutions. Staining for lo minutes in safranin-anilin water solution gives 

 beautiful pictures. After staining, the preparation is rapidly treated 

 with alcohol and xylol, and mounted in balsam. 



Other basic anilin dyes, such as gentian-violet, methylen-blue, etc., 

 may be used ; these stain only the glycogen. The acid anilin dyes do 

 not stain. 



Pyronin Methyl-Green.* — Whitney recommends a 1 p.c. solution of 

 these two pigments, mixed in the proportion of 4 parts of the pyronin to 

 1 part of the methyl-green solution, as an effective double stain for cells 

 and bacteria. 



Methods of Staining the Diphtheria Bacillus.! — J. M. Blumenthal 

 and M. Lipskeron in an interesting and useful contribution on the 

 comparative value of the differential methods for staining the diphtheria 

 bacillus, award the palm to the methods of Falieres and of Ljubinsky. 

 In the former the staining solution is composed of methylen-blue 2, 

 borax 0*5, distilled water 100, absolute alcohol 8 drops. 



After washing in tap-water the stained film is further treated for half- 

 a-minute with a 1 : 1000 aqueous solution of vesuvin. The granules 

 of the bacteria are stained blue, and show up well on the brown back- 

 ground. 



Ljubinsky's method consists in staining the fixed fihn for ^-2 

 minutes with a solution composed of Merck's pyoktanin 0'25; acetic 

 acid (5 p.c.) loo. 



After washing with water the preparation is after-stained for half-a- 

 minute with a 1 : 100 > solution of vesuvin. 



The results are stated to be excellent, but the authors think they 

 have improved on it by substituting chrysoidin for vesuvin, using, 

 however, a solution three times as strong. 



Eleven other methods are described, but for these the original should 

 be consulted. 



Staining Negri's Bodies in Hydrophobia.} — G-. Fasoli adopts the 

 following method. The material is fixed in sublimate solution, and the 

 sections first stained with aqueous eosin. After washing with water 

 they are differentiated with alcohol, made alkaline with a few drops of 

 1 p.c. soda solution. The sections are again washed, and then stained 

 with methylen-blue, until they are of a pale blue colour. After dehy- 

 dration they are cleared up with xylol, and mounted in balsam. 



New Yolk Stain.§ — K. Peter gives the following modification of 

 Spuler's iron cochineal stain. 10 grm. of powdered cochineal are boiled 

 in 250 c.cm. distilled water, and the decoction evaporated down to 



* Boston Med. and Surg. Journal, May 1903. 

 t Centralbl. Bakt., 1^ Abt. Orig., xxxviii. (190f>) pp. 359-G6. 

 t Policlinico sez. Med.. 1904, No. 7. See also Centralbl. Bakt., l ,e Abt. Ref., 

 xxxvi. (1905) p. 385. § Zeitschr wiss. Mikrosk., xxi. (1904) pp. 314-20. 



