ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 765 



Demonstrating the Blastoderm of Polistes pallipes.* — W. S. 

 Marshall and P. H. Dernehl killed the eggs in hot water, and after a 

 few seconds added an equal amount of hot saturated aqueous solution 

 of sublimate. After an immersion of 20-40 minutes the eggs were 

 washed and placed in 70 p.c. alcohol. Another method used consisted 

 in adding to hot sublimate solution an equal bulk of alcohol and pouring 

 the mixture over the eggs, and allowing this to act for 10-20 minutes. ' 



The stains used were iron-hamitoxylin, generally followed by Bor- 

 deaux red, and the safranin-methylen-violet, orange G triple stain. 



Preparing Fasciolaria tulipa and its Larval Excretion Organs.! 

 O. C. Glaser found that the best fixative was Kleinenberg's picro- 

 sulphuric acid. The stains used were borax-carmin, hsemalum, 

 Kleinenberg's hasmatoxylin, and Conklin's modification of Delafield's 

 hematoxylin. In some cases bleu-de-Lyon and eosin in combination 

 were tried. 



There was some difficulty in obtaining thin sections, as dehydration 

 rendered the yolk very brittle. For paraffin sections the best results 

 were obtained by superseding the higher alcohols and xylol with 

 70-80 p.c. alcohol and creosote. This procedure enabled thin sections of 

 a mass containing some 300 eggs to be easily made. 



Demonstrating Neurofibrils.!— G. A. Jaderholm rejects the existence 

 of an endocellular network in ganglion-cells, and shows that the 

 appearances are due to fixation, his view being that the fibrils pass 

 through the cells without inosculating. 



He advocates Bethe's method, which consists in fixing with nitric 

 acid, following this with molybdanate of ammonia and toluidin-blue. 

 This procedure causes little shrinkage, and the appearance of an endo- 

 cellular network is absent. By Donaggio's method, which consists in 

 substituting pyridin for nitric acid as fixative, the cells become shrunken 

 and the appearance of an inosculating endocellular network is produced. 



By combining the two methods, shrinkage and artefacts intermediate 

 in degree were produced. 



Demonstrating the Structure of Red Corpuscles.§— VI. BJzicka 

 washes the air-dried films with a mixture of tap and distilled water in 

 order to remove the haemoglobin. The films are then fixed in saturated 

 aqueous solution of sublimate. After thorough washing in running tap 

 water they are mordanted with 5 p.c. sodium nitrate and then washed 

 again. The films are stained with a mixture of 2 parts of 5 p.c. carbol- 

 fuchsin and 1 part of 1 p.c. aqueous china-blue solution. 



After washing in water the preparations are dried and mounted in 

 Balsam or in cedar-oil. 



Demonstrating Teeth of Mammalian Embryos.||— K. von Korff 

 fixed the material, teeth of embryos of ox and pig, in sublimate, 

 sublimate-alcohol-acetic acid, and in Flemming's fluid. The last two 

 have the advantage of not dissolving out the slight deposit of lime. The 



* Zeitschr. wiss. Zool., lxxx. (1905) pp. 122-54 (2 pis.). 



+ Tom. cit., pp. 80-121 (2 pis. and 5 figs.). 



X Arch. Mikr. Auat., lxvii. (1905) pp. 108-23 (2 pis.). 



§ Tom. cit., pp. 82-102 (2 pis.). || Tom. cit.. pp. 1-17 (1 pi.). 



