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sr.MMAK'Y <)K CURRENT RESEARCHES RKLATIM; TO 



and a little broth taken up by suction on the rubber tube held in the 

 mouth. If the point is sufficiently patent, a little drop should be easily 

 blown out on to the cover-glass. The size of the opening will vary with 

 the nature of the work in 'contemplation. If too large, say over 

 15 micrometers, the difficulty of isolation will increase ; if too small, say 

 less than a micrometer, it will be difficult to blow out broth or introduce 

 the larger bacteria. Bacteria can be isolated by means of the high 

 powers. The tip is brought into the drop near the bacterium and is then 

 lowered, the bacterium usually entering the pipette by capillarity. It 

 can be isolated on a sterile part of the cover-glass. In using cover-glass 



Fig. 8. — Method of making the capillary pipette. 

 b, microburner. 



cultivations, broth, liquefied gelatin, or any fluid or semi-fluid, may be 

 employed. The media may be placed in readiness on the slide previous 

 to isolation. 



The isolated organisms may be inoculated into animals, subcutane- 

 ously, intravenously, or intraperitoneally. The organism is washed well 

 back into the pipette with broth or salt solution. The end of the 

 pipette (which should be obliquely fractured) is inserted into the tissue 

 of the animal and the organism blown out. Large animals may be 

 inoculated through the mucous membrane of the mouth. 



In the latter portion of his paper Barber gives full details of the 

 various special applications of the pipette method that he has employed 



