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srUMAKY OF ( '( l.-KKN I RESEARCHES RELATING To 



appended considerable information not before published regarding the 

 various applications of the method. The technique lias a very wide 



application, not only in bacteriology but also in all departments of 

 microscopy. Single bacteria, yeast-cells, spores of fungi, algae, protozoa, 

 Mood-corpuscles, and other histological elements may be isolated with 

 comparative ease. Isolated organisms may lie cultivated /// situ, trans- 

 ferred to any medium, or inoculated into animals Injections may be 

 made into the vacuoles or protoplasm of living cells. Microscopical 



Fig. 6. — Microscope with two pipette-holders, each containing a 

 pipette attached to the stage by rneaus of metal plates. Seen 

 from the back : A a three-movement, and B a two-movement 

 holder ; tf, adjustment 'governed by screen s" for moving the 

 pipette to and from the observer. 



objects may be dissected or stained under the highest powers of the 

 Microscope. 



The principle of the method consists in the separation of a single 

 organism by means of a very finely- pointed capillary glass pipette. The 

 isolation is carried out in hanging drops on the under-side of a large 

 cover-glass, which is placed over a moist chamber. The organism to be 

 isolated is touched with the tip of the pipette, into which it enters by 

 capillarity ; a sterile portion of the cover-glass is brought over the tip 

 and the organism is discharged on to it by pressure through a rubber 

 tube held in the mouth of the operator. The whole process is carried 

 out under the Microscope, with the highest powers, if desired. 



