ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 625 



superfluous water poured off. The Nematodes were killed and fixed by 

 adding to this watery mud an equal quantity of boiling hot saturated 

 solution of corrosive sublimate. For staining and mounting each 

 sample was treated as follows : The sediment was examined, a little at a 

 time, in a Syracuse watch-glass under a dissecting-lens ; the Nematodes 

 were picked out one at a time with a bamboo splinter and placed in 

 water in the object-box of a differentiator, in which they were gradually 

 passed up through upgraded alcohols to 80 p.c. At this point they 

 were treated with acid-alcohol to dissolve out impurities (10 drops 

 concentrated HC1 to 100 c.cm. 80 p.c. alcohol), and overstained with 

 acid carmine according to following formula : Carmine 4 grm., H 2 

 15 c.cm., HC1 30 drops. Add 95 c.cm. of 85 p.c. alcohol, boil until 

 the carmine is dissolved, neutralize with ammonia until carmine begins 

 to precipitate, filter through glass wool. For differentiation of the 

 tissues acid-alcohol was used (4 drops concentrated HC1 to 100 c.cm. 

 Do p.c. alcohol). The specimens still in the object-box were passed up 

 to and through absolute alcohol and turpentine to thin balsam. This was 

 done without removing the object-box from the differentiator except to 

 remove it to another type of differentiator when the change to heavier 

 fluids began. The object- box was now opened in thin balsam in a 

 Syracuse watch-glass and the Nematodes mounted in balsam. From 

 ten to 100, according to size, can be arranged in one drop of balsam 

 without much crossing of specimens. This is also best done under 

 magnification ; it is convenient to have two dissecting Microscopes, 

 keeping the watch-glass of specimens under one and the slide which is 

 being prepared under the other. 



New Method of Examining Stools for Eggs.* — Vida Annette 

 Latham reports tbat C. M. Fauntleroy and R. Hayden suggest the follow- 

 ing method. 1. Mix thoroughly about 2 grms. of ftecal matter with 

 5 c.cm. of a 2 p.c. aqueous solution of lysol in a centrifuge tube. 

 2. Centrifuge at high speed for one minute, decant the supernatant 

 fluid, and mix a fresh quantity of the lysol solution with the sediment 

 in the tubes. Repeat this step three times. 3. Remove small portions 

 of the centrifuged deposit with a pipette, place on slide, mix a small 

 drop of anilin-gentian violet with the sediment, cover and examine. All 

 eggs, hookworms, etc., stand out very clearly. Everything is stained 

 except the eggs. 



(5) Mounting:, including- Slides, Preservative Fluids, etc. 



Euparal.f — Euparal, says H. L. Wieman, is a mounting medium 

 composed of a mixture of camsal. sandarac, eucalyptol, and paraldehyde, 

 and having a refractive index of 1 " 483.+ It is put up in two forms, the 

 colourless and the green, the latter containing a copper salt which 

 intensifies hematoxylin stains. The colourless is preferable when stains 

 other than hematoxylin are used. The primary advantage of this 

 medium is that it spares delicate objects the usual treatment with absolute 



* Trans. Amer. Micr. Soc, xxxiv. (1915) pp. 54-5. 

 f Trans. Amer. Micr. Soc, xxxiv. (1915) pp. 52-3. 

 % See also this Journal, 1907, p. 501. 



