FRESH -WATER RHIZOPODS. 



Ill 



in a little water, then, when it is well isolated (Fig. 4) I raise 

 the cover, and with a rag I clean the slip to the right of my 

 rhizopod. It only remains to let fall a drop of clean water 

 on to the slip, introduce the rhizopod into it by means of the 

 needle, and cover all again with a fresh cover-glass. 



All these operations, which may appear intricate, are not 

 so really, and only require two or three minutes as a rule, 

 often even less. The animal is then well isolated, and easily 

 examined, and, without any fear of losing it, it is possible 



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6. 



to compress it, so as to drive out the nucleus, detach the scales 

 or break up the plasm, or to keep it living for many hours, 

 and even, by taking care to add water to one of the edges of the 

 cover from time to time, and covering the slide with a large 

 capsule and a wet cloth, to keep the animal for several days, 

 almost indefinitely. 



It is often important also to make sure of the nature of 

 the shell, to destroy the chitinous part, to isolate the siliceous 

 scales of Eughjpha, Nebela, etc., or the needles of Heliozoa, 

 and to do that, I have often found it advantageous to subject the 

 shell either to the action of boiling sulphuric acid, or to the 

 flame of the blowpipe. 



