374 SUMMARY OF CURRENT RESEARCHES RELATING TO 



A piece about the size of a pea is placed between two slides ; by squeez- 

 ing these together two thin even films are produced. Upon the films 

 are arranged the celloidin sections. Upon these are placed several folds 

 of blotting paper, and firm but gentle pressure applied. 



Instead of the foregoing procedure, some of the liniment may be 

 mixed with distilled water to a syrupy consistence, and a film made on 

 the slide with a camel's-hair brush. In any case, it is always necessary 

 to make the smears immediately before arranging the sections, as the 

 linimentum dries very quickly. 



The slides covered with sections are then placed in a vessel contain- 

 ing 96 p.c. alcohol for a quarter to half an hour before they are exposed 

 to any after-treatment, such as staining or mounting. 



If it be desired to remove the celloidin, the slides are immersed in a 

 solution of equal parts of alcohol and ether for half an hour or more, 

 and then transferred to 96 p.c. alcohol. 



Method for Sticking Paraffin Sections to the Slide.* — H. Michaelis 

 places the section in warm water (45°) and removes it thereform on a 

 slide. After removing the superfluous water with blotting-paper, a 

 piece of smooth writing-paper is pressed firmly on the section. On 

 carefully lifting the paper the section is removed along with it. The 

 paper is then cut off all round the section, care being taken not to have 

 any piece projecting beyond the edge of the section. A slide is now 

 covered with a layer of glycerin albumen, and upon this the section is 

 laid, paper side uppermost. After pressing the section firmly down, the 

 albumen is coagulated in the flame. When the paraffin is dissolved out 

 in xylol the paper falls off. 



(4) Staining and Injecting. 



New Method of Staining with Iron Hsematoxylin.f — A. Paine, 

 in a communication made to the Pathological Society of London, 

 recommended the use of iron and hematoxylin in one solution, not 

 in separate and consecutive solutions as in the methods of Heiden- 

 hain and Benda, and without subsequent decolorisation. Such a solution 

 he prepares by adding in certain proportions a 5 p.c. solution of hgeina- 

 toxylin (Grubler) in absolute alcohol, to a weak solution of perchloride 

 of iron, e.g. 1 to 1000 of the B.P. liq. ferri perchlor. fort. Convenient 

 proportions were found to be 5 to 10 drops of the former to 10 c.cm. 

 of the latter solution. This stain can be used after alcohol, mercury or 

 bichromate fixation, but the best results followed fixation in 3 p.c. 

 potassium bichromate and 5 p.c. glacial acetic acid added at the time of 

 using. 



Staining of Bacteria difficult to Stain (Glanders and Typhoid 

 Bacilli, Gonococci, etc.) in Sections of Skin and other Organs.^ — 

 K. Zieler recommends for sections, to be stained with polychrome 

 methylen-blue, a preliminary staining with acid orcein solution. By 



* Centralbl. allgem. Pathol, au. pathol. Anat., xiv. (1903) pp. 264-5. 

 t Lancet, i. (1904) pp. 435-6. 



t Centralb. allg. Path., xiv. (1903) p. 561. See also Centralbl. Bakt., xxxiv. 

 (1904) p. 462. 



