473 SUMMARY OF CURRENT RESEARCHES RELATING! TO 



Fix in absolute alcohol or in saturated aqueous solution of sublimate. 

 Embed in paraffin. Stain the sections for 10 to 15 minutes in 1 to 1000 

 eosin. Pour off excess of eosin and mop up with filter-paper. Stain 

 for 15 to 20 minutes with methylen-blue solution prepared as follows : 

 100 c.cm. medicinal methylen-blue solution in distilled water, 5 c.cm. 

 of 10 p.c. solution of sodium carbonate. Leave in sunlight till deep 

 red colour is seen on shaking. Dilute 25 times for use. Pour off 

 excess of stain and wash rapidly in 70 p.c. alcohol. Transfer to water. 

 If too blue, wash in 0*25 p.c. acetic acid and then in water. Allow the 

 section to dry on the slide. Mount in balsam or keep as a film moistened 

 with cedar oil. 



Bielschowskt, M. — Die Silberimpragnation der Neurofibrillen. 



Neurol. Centralbl, xxii. (1903) pp. 997-1006 (5 figs.). 



(5) Mounting:, including: Slides, Preservative Fluids, &c. 



New Mounting Device. — This apparatus (fig. 75) by "Watson and 

 Sons was exhibited at the May meeting, and was described by Mr. Watson 

 Baker (see ante, pp. 382-3). 



Fig. 75. 



(6) Miscellaneous. 



New Method for Sterilising Vessels.* — Rothenbach states that 

 flasks and other vessels can be effectually sterilised by merely inverting 

 a glass cap over the neck or other opening of the apparatus. 



Differentiation of B. typhosus and B. coli communis by Means 

 of the Photographic Plate .f — W. C. Stevenson claims to be able to 

 differentiate B. typhosus from B. coli communis by means of their action 

 on the gelatino-bromide photographic plate. His method is as follows : 

 Cultures of the bacilli are made in broth, samples of the same broth 

 being used for each culture. After, say, 24 hours, a few drops of each 

 culture are placed upon the sensitive surface of the plate, and spread 

 out so as to wet an area of any desired extent. This is done in a 

 faint red light. The plates are then covered up, and allowed to stand 

 for 40 minutes. They are then developed in the usual way. It will 

 be found that the moistened areas develop with very different densities 



* Deutsche Essigiudustrie, vii. No. 37. See Centralbl. Bakt., 2 le Abt. xii. (1904) 

 pp. 152-3. t Brit. Med. Journ., i. (1904) p. 1004. 



