466 



SUMMARY OF CURRENT RESEARCHES RELATING TO 



tubing and steel regulator, thus reducing the risk of fire to a minimum, 

 and also with electric heater and regulator. 



Fig. 100. 



(4) Staining' and Injecting. 



New Blood Stain.* — E. C. Rosenberger finds that phloxin stains the 

 granules of leucocytes, and recommends the following mixture. Satu- 

 rated aqueous solution of methylen-blue 5 parts, saturated aqueous 

 solution of phloxin 2 parts, alcohol (95 p.c.) 3 parts, distilled water 6 

 parts. The stain should be shaken before using. It works well after 

 fixation by heat, alcohol, or ether and alcohol. Stain 1 to 4 minutes, 

 wash, dry, mount in balsam. 



Staining Blood-films.f — Dr. A. E. von Willebrand has devised the 

 following method for the simultaneous staining of blood-films with eosin 

 and methylen-blue. The solution is composed of equal bulks of 0"5 p.c. 

 eosin dissolved in 70 p.c. alcohol and saturated aqueous solution of 

 methylen-blue. To this 1 p.c. acetic acid is added drop by drop until 

 the eosin staining appears ; as a rule, 10 to 15 drops of the acetic 

 acid are sufficient to effect the development. Before use the solution 

 must be filtered. The films should be fixed by dry heat, by absolute 

 alcohol, or by 1 p.c. formol-alcohol. The staining takes from 5 to 10 

 minutes, during which time it is advisable to heat the staining fluid 

 until it vaporises. Tho erythrocytes are red ; nuclei dark blue ; neu- 

 trophil granules violet; acidophiles red; and plasma granules deep 

 blue. 



Staining Diphtheria Bacilli. J — Dr. H. van de Rovaart finds that 

 Loeffier's alkaline-methylen-blue solution heated for 1-1^ minutes gives 

 better results than the acetic acid solution recommended by Ncisser. 

 He regards the after treatment with 3 p.c. hydrochloric acid alcohol as 

 an unnecessary complication, and advises instead contrast-staining with 



* Philadelphia Med. Journ., vii. (1901) p. 448. See Jouru. Applied Microscopy, 

 iv. (1901) p. 1305. t Deutsche Med. Wochenschr., xxvii. (1901) pp. 57-8. 



X Centralbl. Bakt., 1*> Abt., xxix. (1901) pp. 574-5. 



