ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 



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progress the specimens are smeared on the plate and dried. The 

 plate is then placed in the bath. In about four minutes at 50° C. 

 the specimens are stained, and then the fuchsin is withdrawn through 

 C. Water is then admitted through B, and having been siphoned off 



Fig. 102. 



cfc: 



-I L - 



II 



u 



' — > 



« T «"« 'lie, « 



■f 

 I 



^V«^ ^ V -. V V^ V" 



V ' ' 'v l A '. V'-.' -'- - 



■ *~ ' .V -.-- I -^^ - 



I 



? -* 



Fig. 163. 



two or three times, the preparations are decolorised. The decolorising 

 is performed in glass trays, and when finished, the plate is replaced in 

 the bath and washed. It is then withdrawn, dried, stained with 

 methylen-blue, and examined. 



Ammonium Persulphate as a Decolorising Fluid for Staining 

 Spores and Sputum.* — Dr. E. L. Pitfield recommends a solution of 

 ammonium persulphate which acts by oxidation. To demonstrate spores 



* Philadelphia Med. Journ., vii. (1901) p. 872. 



