144 



representing this larva on the day following (the i6th). and 

 ^S 48 (from photograph) on the i8th, when the pigment 

 appeared somewhat differently arranged, viz., a diffused \-ellow 

 (m which the hidividual pigment cells were indistinct) occurring 

 on head and body to a point half-way between the rectum and 

 the end of the tail. Here the yellow pigment expanded into a 

 band, traversing the dorsal and ventral fins. Yellow pigment 

 also occurred on the anterior part of the yolk and on the rectum 

 down to anus. Black branching cells appeared extending from 

 the margin of the body above and below into the adjacent fins. 

 ■^^1 ^SS' which seems to be the same, was found in a surface 

 tow-net on the 17th September, 25 miles off Cape Point. It was 

 about '/6 mm. in diameter, and had no oil globule. 



Agriopiis spinifer, Smith. 



Some time ago the eggs of Agriopus were procured (znde 

 J\Iarinc Investigations, Vol. II., p. 189), but fertilization was not 

 effected. ]\Iore recently a ripe male and female were found in 

 the same haul, and fertilized eggs were secured (Buftel's Bay, 

 25th November, 1903). The eggs varied from r83 to i"J^ mm. 

 in diameter."* Seven hours after fertilization the germinal disc 

 showed about 30 to 40 cells, and on the following morning, or 

 24 hours after fertilization, the blastopore was about three- 

 quarters over the e^^ (Plate IX, fig. 49). The corrugated 

 appearance noted in the unfertilized eggs formerly presen'ed 

 were readily seen at this and all subsequent stages, and seems to 

 be a characteristic of the &^g. Development seemed to be pro- 

 cteding quite normally, and on the 27th the length of the embryo 

 was about half the circumference of the o.^^, at which stage 

 faint branching pigment cells of a dark colour began to appear 

 all over the yolk. On the following day the embryo had 

 increased in length, and the heart, otocyst, and rudiments of the 

 pectoral fin were evident. On the 29th, however, all the embryos 

 v.ere found to be dead or dying. One was dissected out, and is 

 represented in fig. 50. Dark branching pigment cells occurred 

 over the yolk, along the head and body, and on the pectoral fin. 

 1 lie notochord was multicolumnar and the rectum anterior. 



SPECIES XIX. 



On one or two occasions a few opaque spheres were found in 

 tow-nettings preserved in formahn. They were of a dull white 

 colour, minutely vesiculated, but without any signs of oil globule 

 or embryos, and there was therefore some doubt as to their being 



* .\s there was some doubt as to the maturity of the eggs in tiie first case 

 these dimensions are substituted in the key on page 152 \ox those fir.st given. 



