ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 113 



each 100 c.cm. of the clear bile-salt-lactose-agar add 2 c.cm. of 1 in 

 1000 aqueous solution of brilliant green (extra pure, Grubler) and 

 2 c.cm. of a 1 p.c. aqueous solution of picric acid. The resulting clear 

 bright green agar is poured into plates 17-20 cm. in diameter. After 

 solidification the plates are dried in an incubator. The bile-salt-lactose 

 agar made in bulk may be distributed into flasks, each containing 

 150 c.cm. When required for use, one of these is melted and 3 c.cm. 

 of each of the solutions of the dyes added and well mixed. This amount 

 makes three large plates. After inoculation the plates are incubated at 

 37° upside down. In 21 hours typhoid colonies have a diameter of 

 about 1 mm. ; they are quite transparent and clear, while coli have a 

 dark green spot in the centre. These characters are greatly accentuated 

 in 48 hours. Paratyphoid colonies, colonies of the food-poisoning group 

 and of dysentery- are indistinguishable in their growth from those of 

 B. typhosus. After 48 hours the typical colonies may be fished out and 

 submitted to further examination. 



Rosenthal, G., & P. Chazarain-Wetzel — La culture du Bacille per- 

 fringens dans les cultures sporulees en eau blanc d'eeuf du Bacille anaerobic 

 du rhumatisme aigu ; moyen de differenciation des deux varietes du bacille 

 d'Achalme. G.R. Spc. Biol. Paris, lxvii. (1909) pp. 677-8. 



Sinepf, A., & R. Drosdowitsch — Prof. Dieudonne's Blutkaliagar, ein neuer 

 Nahrboden fur die bakteriologische Diagnose der Cbolera. 



[Confirms the value of this medium for isolating cholera vibrio. 



Centralbl. Bakt., lte Abt. Orig., lii. (1909) pp. 429-31. 

 See also this Journal, 1909, p. 661. 



Thaon, P. — Symbiose de Levure et Oospora dans un cas de langue noire. 



[Gives results of cultivations of these organisms.] 



C.R. Soc. Biol. Paris, lxvii. (1909) pp. 705-7, 



(2) Preparing- Objects. 



Studying the Labyrinth.* — W. Kolmer used monkeys for studying 

 the structure of the internal ear. After the blood had been removed 

 during narcosis the vessels were thoroughly washed out with warm 

 Ringer's fluid. As fixative, Held's fluid was used. This consists of a 

 saturated solution of potassium bichromate, 2 to 3 parts ; 10 p.c. formalin, 

 2 parts ; acetic acid, 1 part. In certain instances, trichlor-lactic acid, or 

 trichloracetic acid and uranium nitrate, and, for smaller objects, osmir 

 acid, were added. The fixation lasted, according to the size of the object, 

 for from 3 to 10 weeks. Then followed decalcification, with 5 p.c. nitric 

 acid, followed by immersion in lithium sulphate 4 p.c. for one day. After 

 this the pieces were washed in running water, and after passing through 

 upgraded alcohols were transferred in the usual way to celloidin ; in 

 this they remained for 8 weeks. The sections were stained with iron- 

 hgematoxylin, after mordanting with iron-alum ; the contrast stain was 

 Rubin. 



Studying the Finer Structure of the Labyrinth of Vertebrata.t 

 H. Held, when examining the development of the organ of Corti and of 



* Arch. Micr. Anat. u. Entwickl., lxxiv. (1909) pp. 259-310 (4 pis.). 



t Abhandl. k. Sachsich. Gesell. ^Wissensch., xxxi. No. 5 (1909) 294 pp. (18 pis.). 



Feb. 10th, 1910 i 



