ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 223 



Spores of Bacillus perfringens.* — B. L. Melikov found that the 

 spores were best cultivated in white of egg broth {bouillon blanc d'ceuf) 

 and stained by Ziehl's method with a weak decoloriser (1 p.c. sulphuric 

 acid). The vitality of the spores is great. In aerobic cultures, mixed 

 with B. coll on agar slopes, they grow in about fifteen days, and in 

 white of egg broth deprived of air for four or five years they may also 

 give colonies. The spores are killed at 100° in a few minutes. The 

 spores are large, oval, and usually central. When small and young the 

 spores stain more deeply than when fully developed. 



Babbeb, M. A. — The Effect on Mice of Minute Doses of B. anthracis. 



Joum. Infect. Diseases, vi. 1909, pp. 634-61. 



Kbuyff, E. de — Les Bacteries thermophiles dans les Tropiques. 



Centralbl. Bakt., 2te Abt., xxvi. (1910) pp. 65-74. 



Rankin, A. C. — Germicidal Action of Metals and its Relation to the Production 

 of Peroxide of Hydrogen. Proc. Boy. Soc, Ser. B, lxxxii. (1910) pp. 78-87. 



Savage, W. G. — Differentiation of Streptococci by means of the "Goat Test." 



Rep. Local Govt. Board, 1909, Appendix B 3, pp. 294-315. 



,, „ Etiology of Paratyphoid Fever and the Belationship of Para- 



typhoid Bacilli to other members of the Gaertner Group. 



Op. cit., Appendix B 4, pp. 316-40. 



YVidal, F., P. Abrami, E. Joltbain, E. Bbissaud, & A. Weil — Sero- 

 diagnostic Mycosique. Applications au diagnostic de la Sporotrichose et de 

 l'Actinomycose. Les coagglutinations et cofixations mycosiques. 



Ann. Inst. Pasteur, xxiv. (1910) pp. 1-33. 



* C.R. Soc. Biol. Paris, lxvii. (1909) pp. 806-7. 



