ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 665 



1 p.c. aqueous solution of chloral hydrate ; the teased-out preparations 

 were mounted in glycerin. 



Vital methylen-blue staining gave excellent results. The animal 

 (sheep) was washed out through a carotid artery with warm saline or 

 Ringer's fluid. Warm 1 p.c. methylen-blue solution was then injected. 

 The fluid was allowed to set for 20 minutes and then the eyeball-muscles 

 were removed. The muscles were stretched out on a plate and exposed 

 to light in a moist chamber for £ to 2 hours. The stain was fixed in 

 10 p.c. aqueous solution of ammonium molybdate for 24 hours. They 

 were then washed in distilled water for several hours, after which they 

 were dehydrated rapidly in alcohol, cleared up in xylol and mounted 

 in balsam. 



The methods of Eamon y Cajal and of Bielschowsky were also tried ; 

 the results were about the same, but not so satisfactory as those already 

 given. 



The materials fixed in formalin were imbedded in celloidin, paraffin, 

 or paraffin-celloidin, and the sections stained with 

 haemalum, iron-ha3inatoxylin, and counter-stained 

 with picro-fuchsin or with one of the numerous 

 preparations of carmin. 



(3) Cutting 1 , including- Imbedding and Microtomes. 



Flatters and Garnett's " Firmax " Micro- 

 tome.* — This instrument (fig. 97) has been de- 

 signed in order to meet the demand for a low- 

 priced efficient microtome. It is substantially 

 made with thick brass knife-plate and special 

 arrangements for preventing the wax from turn- 

 ing round. It is provided with table-clamp and FlG 97 

 thickness register. . 



"&■■ 



Van der Stad's Improved Rocking-microtome.t — The description 

 of this instrument is due to J. Boeke. Fig. 98 gives the general view, 

 which, it will be noticed, differs from the standard type of rocking- 

 microtome rather in elegance and stability than in essentials. The author 

 includes the following among his improvements. 



1. One of the four feet of the heavy base-plate A is shorter than 

 the other three, and serves for the reception of a position-screw (a, figs. 

 D8 and 102). 



2. The instrument can, at desire, be arranged (fig. 99 RMl) for 

 section-thicknesses from 0-25 /*, proceeding by 1 n ; or for section- 

 thicknesses (RMn) from 0-20 fx, proceeding by '• 5 /*. 



3. The object-holder (figs. 98, 100,101) has some special advantages, 

 in addition to the ordinary movements, in three mutually perpendicular 

 planes for setting the object with any desired orientation. The upper 

 part (figs. 98 and 100) of the holder consists of a strong angle-piece c, 

 the shorter side of which carries a strong flattened rounded plug, which 



* Catalogue B, 1910, p. 33. 



t Zeitschr. wiss. Mikrosk., xxvi. (1909) pp. 242-55 (6 figs.). 



Oct. 19th, 1910 2 Y 



