256 SUMMARY OF CURRENT RESEARCHES RELATING TO 



(4) Staining and Injecting. 



Method of Staining Peripheral Nerves.* — T. Maruyaina discusses 

 the method devised by Yamagiwa and its applicability to pathological 

 tissues, more particularly in connection with the study of beri-beri. The 

 process is as follows : — After hardening in Muller's fluid in the ordinary 

 way and imbedding in celloidin, sections are cut. They are stained in 

 concentrated alcoholic eosin for a period of from 1 to 12 hours. Next, 

 after prolonged staining with concentrated watery anilin-blue, they are 

 placed in a differentiating fluid — weak alcohol make slightly alkaline by 

 the addition of liquor potassa? — and washed in distilled water. The 

 sections are then put into weak alcohol to remove excess of anilin-blue, 

 dehydrated in absolute alcohol, cleared in oil, and mounted in balsam. 

 In sections stained thus, the axis-cylinders appear deep blue, medullary 

 sheaths red, connective-tissues and cell-nuclei bright blue, red blood- 

 cells pink, and unstriped muscle pale violet. The preparations lose their 

 stain usually in a few months. 



Fluoride of Silver in Golgi's Method.| — E. Saragnone describes 

 a new procedure for demonstrating the intracellular network. It is 

 really a modification of Golgi's method, fluoride of silver being sub- 

 sisted for the nitrate. The preparation used is known as " tachiolo 

 Paterno," and is a 10 p.c. solution of silver fluoride, which is not 

 reduced by the action of light. The full procedure is as follows : 

 1. The pieces are fixed in a solution consisting of formalin (20 p.c.) 

 30 grin. ; saturated solution of arsenious acid, 30 grm. ; alcohol (96 p.c.) 

 30 grm. Time, 10 to 12 hours. 2. The pieces are then transferred to 

 tachiolo Paterno, 30 c.cm. ; distilled water, 100 ecru., for one or two 

 hours. 3. They are next washed quickly in distilled water, after which 

 they are immersed for a few minutes to an hour in hydroquinone, 

 30 grm. ; sulphite of soda, 5 grm. : formalin, 50 grm. ; water, 1000 c.cm. 

 4. After- washing in distilled water the pieces are passed through up- 

 graded alcohols to xylol and imbedding. 5. The sections are treated 

 with the following solutions mixed immediately before use : (a) hypo- 

 sulphite, 30 grm. ; sulphocyanide of ammonium, 30 grm. ; water, 

 1000 grm. ; (&) chloride of gold, 1 grm. ; water, 100 grm. The 

 reaction is watched and suspended when the sections have assumed a 

 definite grey tint. 6. Wash in distilled water and pass rapidly through 

 permanganate of potassium, 0*5 grm. ; sulphuric acid. 1 grm. ; distilled 

 water, 1000 grm. 7. Wash rapidly in 1 p.c. solution of oxalic acid, 

 and afterwards in distilled water. 8. Stain with carmalum. Wash 

 again. 9. Pass through alcohol and mount in balsam. 



Studying the Development of Crucifera4 — R. Vandendries fixed 

 the material for a day or so in Bouin's fluid, then washed it till it was 

 white in one-third alcohol, and afterwards preserved it in 80 p.c. alcohol. 

 Sections, 8-12 fi, were stained preferably by Heidenhain's method. 

 As the slow method was found not to be particularly suitable for 



* Mitt. Med. Fakul K.-Jap. Univ., viii. 3 (1909) pp. 368-70. 



f Pathologica, i. (1909) pp. 536-8. 



X Zeitschr. wiss. Mikrosk., xxvi. (1909) pp. 422-4. 



