ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 479 



results. The others were observed up to the end of about three months 

 without any signs of tuberculosis being observed. The author remarks 

 on the fact that he did not succeed in demonstrating the presence of 

 acid-fast bacilli other than the tubercle bacilli in the organs of any of 

 the animals examined post-mortem, and he attributes this fact to the 

 freedom of the inoculated material from fat. 



Tuberculosis in Cold-blooded Animals.* — Herzog investigating 

 experimental tuberculosis in cold-blooded animals, showed that the 

 pathological manifestations produced in the body of the frog as the 

 result of infection by the bacillus of mammalian tuberculosis were iden- 

 tical, both naked-eye and microscopically, with those initiated by the 

 bacillus of fish tuberculosis, the seat of inoculation usually employed 

 being one of the lymph-sacs or the peritoneal cavity. Microscopical 

 sections of tuberculous nodules from the frog's kidney demonstrate this 

 point in a most striking manner. He further states that the bacilli 

 introduced soon disappear from the seat of inoculation, and may finally 

 be demonstrated in all the internal organs. The bacillus of mammalian 

 tuberculosis after passage through cold-blooded animals loses its viru- 

 lence for warm-blooded animals, so that fatal infection of guinea-pigs 

 can no longer be produced even when large doses are employed. A list 

 of the literature of the subject is appended to the paper. 



Pseudo-Tuberculosis (Streptobacillary) of the Grey Rat.j — At 

 the post-mortem of an experimental rat, killed by the administration of 

 terebinthine, Sabrazes observed suppurative lesions limited to the liver 

 and lungs. In the liver these consisted of a lenticular granulation filled 

 with greyish pus, and in the lungs and pleurae of small tubercles filled 

 with greenish-yellow pus. The pus contained numerous rods (8-11 ju- 

 by 0*35 /a) arranged end to end, slightly curved, not branched, non- 

 motile, staining well with the ordinary anilin dyes, but decolorised when 

 treated by either the Ziehl-Neelsen or Gram's methods. Planted on 

 agar at 37° C, the pus gave a pure culture of this streptobacillus, which 

 in plates formed circular colonies, yellowish by transmitted light, at- 

 taining a maximum diameter of 1 mm. Agar streak, a slightly raised 

 transparent streak of the colour of the medium, with sinuous borders 

 showing a more or less well-marked double contour. Inspissated blood- 

 serum and glycerin-agar show similar appearances. Broth becomes 

 uniformly turbid, reaction unchanged ; later a light pellicle is formed, 

 which eventually breaks up and sinks with the rest of the growth to the 

 bottom of the vessel, the bulk of the medium again becoming clear. 

 Milk is not coagulated. Upon potato a scanty growth consisting of a 

 greyish-white layer takes place. On gelatin at the room temperature 

 there is no liquefaction of the medium. The bacillus does not ferment 

 sugar, form indol or spores, or possess nagella ; its virulence is lost and 

 its vitality is slight, subcultivation is necessary about every eighth day 

 to ensure growth ; it does not grow well anaerobically. In culture it 

 forms filaments from 5-60 /x in length, composed of unequal rods or 

 even cocco-bacillary forms. In old cultures granular and swollen 

 involution forms appear. 



* Ceutralbl. Bakt., I' 9 Abt., xxxi. (1902) pp. 78-85. 

 t Ann. Inst. Pasteur, xvi. (1902) pp. 97-105. 



