622 SUMMARY OF CURRENT RESEARCHES RELATING TO 



(4) Staining- and Injecting-. 



Staining Biliary Canaliculi.* — St. CiecLanowski recommends Wei- 

 gert's nerve-staining method for demonstrating biliary canaliculi. Small 

 pieces of liver are fixed for not less than 24 hours in 2-4 p.c. formalin, 

 and after-hardened in alcohol. The sections are mordanted in 0*5 p.c. 

 chromic acid solution for 2 hours, and then stained in Weigert's hema- 

 toxylin solution. When sufficiently stained the sections are differentiated 

 in the ferridcyanide solution, and having been thoroughly washed are 

 mounted in the usual way. 



Glycogen Staining. f — Best publishes a method for staining glycogen 

 in the liver, tumours, &c, by means of lithium-carmin. The procedure 

 is as follows. The sections are first stained with Delafield's or Bohmer's 

 haematoxylin, and after having been washed in water are immersed for 

 15-20 minutes in a freshly made mixture consisting of carmin solution 

 2, liq. ammon. caust. 3, and methyl-alcohol 6. 



The carmin solution is prepared by boiling a mixture of carmin 1, 

 ammon. chloratum 2, lithium carbonicum 0*5, and 50 water. To this, 

 when cold, 20 ccm. of liq. ammon. caustici are added. After the carmin 

 staining the preparations are decolorised in the following mixture, fre- 

 quently renewed : — methyl-alcohol 2, absolute alcohol 4, water 5, or in 

 liq. ammon. caustic. 1, absolute alcohol 2. Dehydration in 80 p.c. and 

 100 p.c. alcohol, oil, balsam. 



The tissue should be fixed in absolute alcohol and imbedded in 

 celloidin. 



(5) Mounting-, including- Slides, Preservative Fluids, &c. 



Sodium Silicate as a Mounting Medium for Microscopical Pre- 

 parations.:}: — Schiirhoft recommends silicate of sodium mixed with 

 10 p.c. glycerin and 10 p.c. water for mounting microscopical prepara- 

 tions. 80 parts of commercial silicate of sodium solution are added 

 to a mixture of 10 parts glycerin and 10 parts previously mixed. The 

 medium hardens well in the course of a few hours. 



Simple Method for Preserving Urinary and other Deposits.§ — 

 K. Bohnstein says that the deposit or sediment from secretions or dis- 

 charges may be preserved in the following simple manner. In the case 

 of urine the sediment is first treated with an equal bulk of 2 p.c. 

 formalin, and the deposit therefrom mixed with an equal volume of the 

 following solution : — formalin 20, glycerin 125, distilled water up to 

 200. When the material to be preserved is of a more solid character 

 such as vomit or faeces, it is thoroughly mixed with an equal bulk of 

 the solution. 



(6) Miscellaneous. 



Micrometer Gauge. || — This micrometer gauge (fig. 125), made by 

 Elliott Brothers, is excellently adapted for ascertaining the thickness of 

 cover-glasses. The instrument is fitted with a ratchet head to prevent 



* Anat. Anzeig., xxi (1902) pp. 426-30. 



t Deutsch. Med. Wochenschr., 1902, No. 5, Vereins-Beil., p. 36. 



1 Deutsch. Apoth.-Zeit. See Zeitachr. angew. Mikr., viii. (1902) p. 54. 



§ Fortschr. d. Med., xx. (1902) pp. 41-4. 



!| Elliott Bros.' Catalogue, 1902, p. 18, fig. 3550. 



