248 



SUMMARY OK OUJUliENT RESEARCHES DELATING TO 



mid examined microscopically and subcultured to determine whether the 

 organism is obligate anaerobe or not (fig. 56). 



2. By the method of J. H. Wright, an ordinary test-tube containing 

 8-10 c.cm. of some fluid medium is inoculated, and a sterile plug of 

 wool is pushed down in such a way as to touch the medium ; on to this 





Growth 

 free 

 zone 



«§§r* 



Growth 

 free 

 zone 



Rubber stopper 



Fin. 56. 



plug sodium pyrogallate solution is dropped, and the tube is at once 

 closed with a rubber cork. A refinement of this method was devised 

 by Burri, who flamed the wool plug lief ore it was pushed into the tube, 

 and after it had been pushed down a second wool plug was introduced, 

 and this was soaked with the pyrogallate solution, the tube being then 



