ZOOLOGY AND BOTANY, MICKOSOOPV, KTC. 



249 



closed with a rubber cork, thus avoiding much risk of contaminating 

 the medium (fig. 57). 



This modified method is also applied to plate cultivations ; a small 





— Rubber stopper 



Hygroscopic 

 cotton wool 

 stopper saturated 

 with alkalin 

 pyrogallol 



Fig. 57. 



Sterile dry plug of 



non-hygroscopic 



wool 



Inoculated 

 medium 



3 cm.. 



Fig. 58. 



Rubber stopper 



Hygroscopic 

 cotton wool 

 stopper saturated 

 with alkalin 

 pyrogallol 



Sterile dry plugof 



non-hygroscopic 



wool 



A Anaerobe plate 



glass dish 80 by 80 by 7 mm. being used to hold the medium, and 

 which, after inoculation, is passed into the tube, which is plugged and 

 corked as before (fig. 58). 



The author also describes a method for cultivations under conditions 

 completely free from oxygen. The apparatus is shown in tig. 59 ; it 

 consists of a long tube holding sterile broth, and communicating at the 

 middle with a short tube, in which is the inoculating material, and both 

 tubes are corked, like the modified Wright's tube (fig. 57) ; after stand- 

 ing at 37° C. for five days, the long tube is inoculated, and after 18 hours 

 the broth is clouded. The absence of oxygen is demonstrated by control 

 tubes, the long arm containing a clear solution of pyrogallic acid, the 



April 15th, 1908 s 



