ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 



255 



further thinned down by means of the first-mentioned method, and 

 when of suitable thickness may be mounted straight away or first stained 

 with a 1-3 p.c. eosin solution for the purpose of detecting traces of 

 organic matter. Several pages full of precautions to be taken during the 

 different stages are given, but for these details the original should be 

 consulted. 



(4) Staining: and Injecting-. 



Staining the Tubercle Bacillus.* — M. Herman recommends the 

 following method as being superior to the Ziehl-Nielsen procedure. 

 He uses a 1 p.c. solution of ammonium carbonate in distilled water as 

 a mordant, and a 3 p.c. solution of crystal-violet (methyl-violet 6 B) 

 in 95 p.c. ethyl-alcohol. The solutions are mixed when required for 

 use in the proportion of 3 of mordant to 1 of stain. The sections or 

 smears are hot-stained in the usual way and then decolorised with 10 p.c. 

 nitric acid and 95 p.c. alcohol. The author claims that by this method 

 many more tubercle bacilli are to be demonstrated than by any other. 



Syringe for the Injection of Lymph-vessels, f — P. Bartels gives 

 the following description of a syringe (fig. 63) used by him for anatomical 



Fig. 63. 



purposes, and especially for the injection of lymph-vessels : A. The 

 syringe barrel (1) consisting of a graduated glass tube, having at one 

 end (2) a metal nozzle, and at the other end (3) a metal ring, both 

 being provided with a knob for a bayonet lock. B. A metal club con- 

 sisting of a rod (4) and a piston (5) in the middle of which a ring is 

 cut out for a washer. C. A metal junction piece (9) fitted to the 



* Ann. Inst. Pasteur, xxii. (1908) pp. 92-6 (1 fig.), 

 t Anat. Anzeig., xxx. (1907) p. 613 (1 fig.). 



