514 SUMMARY OF CDRKKNT RESEARCHES RELATING TO 



jaws in Mo by means of an alcoholic borax-carmin solution (4-G days). 

 The material was then dehydrated in upgraded alcohols and imbedded 

 in paraffin, the intermediary being cedar oil. The sections varied from 

 20-25 fi in thickness. If the borax-carmin had not been successful the 

 sections were also stained with bleu de Lyon. 



Fixation with Trichloracetic Acid and Uranyl Acetate.* — H. 

 Friedenthal praises the action of a mixture of uranium acetate and 

 trichloracetic acid for fixation purposes. Excellent results are ob- 

 tainable from a fluid composed of equal parts of saturated uranium 

 acetate solution and 50 p.c. trichloracetic acid. As a universal fixative 

 which is said to satisfy the requirements of botanists and zoologists 

 alike, a solution with the following composition is given : — Trichlor- 

 acetic acid 20, uranium acetate 10, chromic acid 1, osmic acid 0'5, 

 platinum chloride ■ 5. 



Studying the Histogenesis of Cysticercus pisiformis.t — R. T. 

 Young obtained his material by feeding young Lepus cuniculus (Belgian 

 hare) and Lepus pinetis with proglottids of Tcenia serrata. The liver, 

 omentum, lungs, and mesenteric glands were found infected. The best 

 fixative was Flemming's strong chrom-aceto-osmic mixture, in which 

 the larvae were immersed for two to three hours. After washing in 

 running water, they were passed through up-graded alcohols. The next 

 best fixative was saturated sublimate in 70 p.c. alcohol, to which 1 p.c. 

 glacial acetic acid was added. 



Heidenhain's iron-haamatoxylin, sometimes used with no counter- 

 stain, but more often in conjunction with eosin, Bordeaux-red, or satu- 

 rated aqueous solution of water-blue and picric acid, gave the best results 

 in staining. Vom Rath's, Apathy's, and Golgi's methods were also tried, 

 but none gave very satisfactory results. 



Examining the Neuro-epithelium of the Auditory Apparatus. — 

 N. van der Stricht: used bat-embryos chiefly, also those of guinea-pigs, 

 cats, and one human embryo. This material was fixed in Flemniing 

 (2-4 weeks), Hermann (8 days), acetic-sublimate alcohol (1 day), Perenyi 

 (1 hour), Bouin (1-2 days) ; Benda's method of fixation was also tried, 

 and found to give excellent results. On the whole, the fluids which con- 

 tained osmic acid gave the best results. Material when fixed, if left in 

 iodine-alcohol (70 p.c.) for 5 months to 2 years, was found to stain 

 intensely by the iron-alum method. The cochleas were decalcified in 

 3 p.c. nitric acid and afterwards imbedded in paraffin by means of the 

 disulphide method. Pieces fixed in fluids not containing any osmic acid 

 were stained en bloc in borax-carmin. The sections were mostly stained 

 with iron-haernatoxylin and Bordeaux red. 



Examining the Tentacular Apparatus of Cephalopods.§ — J. Guerin 

 fixed the material in Flemming's, Bouin's, or Carnoy's fluids. In the 



* S.B. Gesell. Natur., Freunde, Berlin (1907) pp. 207-11. 



t Zoolog. Jarhb., xxvi. (1908) pp. 183-254 (4 pis.). 



X Arch, de Biol., xxiii. (1908) pp. 541-693 (5 pis.). 



§ Arch. Zool. Exper. et Gen., viii. (1908) pp. 1-178 (4 pis.). 



