ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 781 



(6) Miscellaneous. 



Pipette for Microscope Work.* — M.Wolff describes a pipette (fig. 172) 

 which he has found useful in Microscopical work. It is made on the lines 

 of the Stroschein syringe, and consists of a glass tube of 4 mm. bore which 

 is provided with a point in the usual way, whilst at its upper eud it has 

 two welts by means of which it may more conveniently be held between 

 two fingers. The aspirator consists of a small cylinder of a bore of 

 5 * 5 mm. and 40 mm. long, and is hermetically sealed at its upper end. 



The open end of the cylinder has a piece of rubber tubing 1 cm. 



£ i Eir/ ar.Ri iu 



3SS 



Fig. 172. 



long, 4 mm. bore, and <s mm. external diameter slipped over it. The 

 tubing is accordingly narrower than the body of the pipette. A length 

 of 7 mm. of the rubber tube is slipped over the cylinder and the remainder 

 embraces the pipette so that an airtight joint is insured. 



The pipette is charged by drawing the cylinder up. The pipette 

 should be held between the thumb and the middle finger, and by slowly 

 pressing upon the cylinder with the index finger the fluid is very easily 

 ejected in single drops. The apparatus has been placed on the market 

 by E. Leitz, of Berlin. 



Mesophotography and its Application to Delicate Unfixed 

 Embryos.^ — C. J. Patten defines mesophotography as the photography 

 of objects of natural size, or but slightly enlarged or reduced. The 

 apparatus used consists of a camera with an ordinary front, but arranged 

 to take different sized lenses by a series of adapter flanges. The lens 

 used was a Zeiss microplanar of 75 mm. focal length. Most of the 

 photographs which the author took were of embryos which five minutes 

 before were within the uterus of the living parent. Having detached 

 the embryo from the uterus of a freshly killed animal, all that has to 

 be done is to fill a glass capsule with cold distilled or boiled water, drop 

 in the embryo, place the capsule on the stand in a position under the 

 lens, bring the embryo into the field with a touch of a soft camel hair 

 brush, focus it, cap the lens, draw the dark slide, wait a tVw seconds 

 until all objects seen reflected in the water appear perfectly motionless, 

 remove the cap gently, and expose the plate. 



The advantages claimed for this procedure are its simplicity, 

 rapidity, and usefulness for making illustrations of the external form of 

 the embryo for plate reproductions. 



* Centralbl. Bakt,, lte Abt. Orig., xlvi. (1908) p. 648 (1 fig.), 

 t Brit. Med. Jouru. (190S) ii. pp. 593-4. 



