ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 107 



B. Technique.* 

 (1) Collecting- Objects, including: Culture Processes. 



Cultivation of Human and Bovine Tubercle Bacilli, f-- W. K. 

 Park and 0. Krumwiede, who have been investigating the relative 

 importance of the bovine and human types of tubercle bacilli in the 

 different forms of human tuberculosis, state that all cultures were iso 

 lated by means of the guinea-pig, and finding that egg-media were 

 eminently successful, used them in the following two combinations : (1) 

 Dorset's medium, the whole egg mixed with 10 p.c. water ; (2) Lubenau's 

 medium. 10 eggs mixed with 20o c.cm. of glycerin-bouillon. It was 

 found that the human virus grew better from the start on the glycerin- 

 egg medium, while the bovine variety was inhibited. They arrive at 

 the following general conclusions : (1) All cultures growing luxuriantly 

 on glycerin-egg from the start are of the human type. (2) All cultures 

 growing sparsely (or even not at all) on glycerin-egg in the first few 

 generations are of the bovine type. 



Method of Isolating and Growing the Lepra Bacillus of Man.i 

 F. W- Twort started from the idea that, as there may be a close relation- 

 ship between tubercle and leprosy, the leprosy bacillus might be culti- 

 vated on media to which tubercle bacilli had been added. Accordingly*, 

 pure cultivations of tubercle were obtained, and the bacilli were ground 

 up with glycerin and saline, and having been steamed for half an hour 

 were added to the yolk and white of new laid eggs in the following 

 proportions : Eggs 75 parts, 8 p.c. sodium chloride 25 parts, tubercle 

 bacilli 1 p.c, glycerin 5 p.c. or less. The medium was placed in test 

 tubes, heated to 60° C. for 1 hour : on the following morning incubated 

 at 38° C. for hours, and again heated in a water bath at 60° C. for 

 1 hour, and set in slopes at 85° C. 



The ericolinized nasal discharge of a leper § was inoculated into this 

 medium, the tubes being capped with rubber, and incubated at 38° C. 

 After 2-1 hours the medium absorbed a quantity of the ericolin, so that 

 the material was lifted off with a platinum loop and rubbed over fresh 

 tubes. The bacilli grew and were subcultured in pure growth. The 

 bacilli were fairly long-headed rods, and quite acid-fast. The growth at 

 first was extremely slow, and only evident to the naked eye after about 

 six weeks as a colourless film along the needle track. 



Artificial Cultivation of Animal Tissues. |j — M. T. Burrows and 

 A. Carrel give, in a series of communications, an account of their experi- 

 ments, which show that portions of various animal tissues can be removed 

 from their natural environment and cultivated in an artificial medium 



* This subdivision contains (1) Collecting Objects, including Culture Pro- 

 cesses ; (2) Preparing Objects ; (3) Cutting, including Embedding and Microtomes ; 

 (4) Staining and Injecting ; (5) Mounting, including slides, preservation fluids, etc. ; 

 (6) Miscellaneous. 



t Centralbl. Bakt., lte Abt. Ref., xlvii. (1910) pp. 673-80. 



X Proc. Roy. Soc, lxXxiii. (1910) pp. 156-8. 



§ For the Ericolin method see this Journal, 1909, p. 526. 



|i C.R. Soc. Biol. Paris, lxix. (1910) pp. 291-4, 298-301, 328-34, 365-8. 



