Method of Disintegrating Bacteria. By J. E. Barnard, 597 



soft iron is fixed, and this is kept from rotating by means of the 

 electro-magnets J. The only constructional difference is that the 

 containing cylinder F is made of vulcanite so that it is perfectly 

 diamagnetic. The chief advantage of this design over the one 

 previously described is that it can be completely covered by a 

 glass bell-jar while in action. A bactericidal agent may be placed 

 in the groove 0, and the bottom edge of a bell-jar allowed to dip 

 into it. 



The actual effect of the process may be seen in the illustrations 

 (Plate XVIII. figs. 1 to 6). 



Fig. 1 is a photograph of some bacterial cells before grinding ; 

 figs. 2 and 3 being the same cells after grinding for fifteen and 

 thirty minutes respectively. 



Fig. 4 is a photograph of unground yeast ; figs. 5 and 6 show- 

 show the result of grinding for fifteen and thirty minutes re- 

 spectively. 



It will be seen in the case of the yeast preparation that after 

 the thirty minutes' grinding the cell contents have been completely 

 expressed. This shows itself clearly, as the cell bodies are un- 

 stained, and show up lighter on the stained cell contents, while in 

 the unground yeast the contrary is the case. 



