46 



Helozoa ... ... ... ... ... ... Mr. James Russell. 



Pediculus vestimenti ... ... ... ... Mr. W. Smart. 



yy SCd'OBl/ ••• •»• ••• hi n 



Volcanic ash from Charn wood Forest... 

 Phonolite from Cornwall 

 Leucite and Dolor ite from Eifel 

 Trachyte ,, ,, „ Rhine 



Trans, sec. spinal cord of Calf 



„ „ Human Kidney 

 Planorbis corneus 

 Cholestrin from alveolar abscess ... ... „ „ 



Foraminifera. Lagena... ... ... ... Mr. A. C. Tipple. 



Ichneumon fly, Diapria ... ... ... Mr. J. J. Vezey. 



Saprolegnia Gahbardensis ... ... ... Mr. J. G. Waller. 



,, Varniensis ... ... ... ... „ ,, 



Crystals, Platino-cyanide of Strontium ... Mr. J. Willson. 



June 13th, 1884. — Conversational Meeting. 



The sixth and last of the second series of demonstrations " On Staining 

 Vegetable Tissues," which was to have been given by Mr. Gilburt, was 

 taken up by Mr. W. Dalton Smith. 



After remarking that he had been somewhat hastily called upon to 

 supply the place of Mr. Gilburt, whose absence by reason of illness all 

 must regret, Mr. Smith proceeded substantially as follows : — 



"Staining, as applied to Vegetable Tissues, is used for three chief pur- 

 poses — 



1st. For rendering objects, which would otherwise be too transparent, 

 more distinct, e.g., cell-walls. 



2nd. For differentiating one tissue from another, e.g., cells from vessels. 



3rd. For differentiating the protoplasm from the formed material. 



The various kinds of stains employed for. these different purposes may 

 be conveniently grouped in accordance with the end in view, thns : — 



1st. For rendering cell-walls, &C, more distinct, the single stains, log- 

 wood, carmine, or one of the aniline dyes. 



2nd. For differentiating various tissues, double stains, of which tho 

 more usually employed are carmine and aniline green, picro-carmine, and 

 magenta and aniline blue. 



3rd. For staining the protoplasm only, Dr. Beale's carmine solution is 

 usually employed. 



It is not my purpose to describe all these processes to-night, since 

 many of them have been fully treated of already; notably, single staining 

 in aniline dyes, and double staining in magenta and aniline blue, by Mr. 

 Gilburt, in a paper read before this Club May 25th, 1877; and double 

 staining in picro-carmine by Mr. Stiles, in the " Northern Microscopist " 

 for July, 1881. Dr. "Bealo, too, in " How to Work with the Microscope," 



