ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 



125- 



Apparatus for the Cultivation of Anaerobic Bacteria without the 

 use of Inert Gases.* — The development of anaerobes in fermentation 

 tubes suggested to Dr. Th. Smith their cultivation in flasks. The appa- 

 ratus depicted in fig. 28 was employed for the cultivation of the tetanus 

 bacillus. There are two bulbs, A and B, connected by a heavy rubber 

 tube C, and with a clamp D to regulate the communication betwoen 

 them. The bouillon ordinarily fills A and the space below the dotted 

 line in B ; but during sterilisation it is forced over into B. It is in- 

 oculated through the opening at E. The growth will extend around to 

 A within 24 hours. F is a tin rack for supporting the apparatus. 

 Pig. 29 shows a variation in which a litre flask A and a bent 100 ccm^ 



Fig. 2it. 



Fig. 28. 



pipette B are used. The upper part of the pipette is shortened and 

 plugged with cotton, while the lower part is bent and fitted to the flask 

 with a rubber stopper. The bouillon is inoculated through D, and the 

 growth reaches the flask in 24-36 hours. This form is only partly 

 filled when sterilised, the extra bouillon being autoclaved separately. 



Urine-Gelatin for the Diagnosis of Typhoid-f — Dr. H. Witticb 

 records some observations made on typhoid material with Piorkowski's 

 medium^ the results of which indicate that the inventor took a too 

 florid view of the value of urine-gelatin for diagnostic purposes. The 

 author has found that, while the appearances of typhoid are as described 

 by Piorkowski, other forms present the same appearances. As a culti- 

 vation medium for typhoid its value is highly appreciated. 



* Journ. Bost. Soc. Med. Bci., iii. [(1899) pp. 340-3. See Journ. App. Micr., ii. 

 (1899) p. 572 (2 figs.). 



t Centralbl. Bakt. u. Par.. l te Abt., xxvi. (1899) pp. 390-6. 

 X Cf. this Journal, 1899, p. 318. 



