264 



SUMMARY OF CURRENT RESEARCHES RELATING TO 



off' the columns, turned through 180°, and replaced ; the second face is 

 then cut, and is parallel to the first. Then the plate s is rotated through 

 90°, and the other two faces are similarly cut. 



_When the paraffin-block has been so " defined," the imbedding plate 



Fig. 68. 



with the block is removed and placed in the clamp of the microtome. 

 The apparatus is made by A. Fromme, Hainburgerstrasse 21, Vienna iii. 



(4) Staining- and Injecting-. 



Staining Malaria Blood.* — Prof. A. Celli stains malaria blood by 

 first fixing the films 15-20 minutes in absolute alcohol. The films are 

 made by allowing the blood to spread itself out between two cover- 

 glasses. The staining is done by the Tiemann-Eemanowsky method : — 

 (a) A saturated aqueous solution of medicinal methylen-blue at 25°-30° 

 for three days ; (b) an aqueous 1 per cent, solution of eosin, A, G. or 

 B (Lucius). One to 3 parts of (a) are mixed with 3-5 parts (&). The 

 time required for staining the films is 20-30 minutes. The red cor- 

 puscle is stained pink, the haemosporidium blue, and its chromatic 

 substance purple. 



Method for Staining the Nuclei of Endoglobular Parasites of 

 Birds.f — M. A. Laveran uses a solution of methylen-blue prepared by 

 Dr. Borrel in tbe following way. A solution of silver nitrate is treated 

 with soda solution, and the precipftated silver oxide carefully washed. 

 To the silver oxide is added a saturated solution of methylen-blue, and 



* Brit. Med. Journ., 1900, i. p. 304. f O.K. Soc. Biol, vi. (1899) pp. 249-52. 



