

ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 



729 



the mass gradually hardens and may be preserved for an indefinite 

 period. Objects prepared by tbe last procedure may be transferred to 

 anise oil, to paraffin, to cedar oil for dry cutting, or to 80 p.c. alcohol 

 for wet cutting. 



Method of Orienting and Imbedding in Paraffin. * — Mr. M. T. 

 Denne describes a process which allows of a number of objects being 

 oriented rapidly and fixed in position before infiltration. The imbedding 

 is done in postal or specimen tubes, and the object is cemented to the 



Fig. 202 



d 



X 



OJytkt 



centre of a paper disk rather less in diameter than the bore of the tube, 

 and carried when in the bath by a wire holder. The cement is a solution 

 of celloidin in equal parts of ether and alcohol, to which as much oil 

 of cloves is added as will give the mixture the consistency of thick 

 honey. The holders are made of brass wire, bent twice at right angles, 

 and curved at the upper end to form handles. A thin sheet-brass disk 

 loosely fitting the tube is soldered between the angles. The piece is 



* Journ. Applied Microscopy, iii. (1900) pp. 888-90 (1 fig.). 



