732 



SUMMARY OF GUEEENT EESEAECHES EELATING TO 



can be easily obtained by simply heating the solution gently several 

 times. Precipitation is avoided by freely diluting the staining solution. 

 By the addition of eosin (in quantity more than half necessary for satu- 

 ration) the stippling of the red corpuscles affected by parasites was 

 brought out. 



New Method for Staining Tubercle Bacilli.* — Dr. E. C. Eosen- 

 berger advises a moderately thick film of sputum which is stained in 

 the usual way with phenol-fuchsin. The film is then contrast stained 

 and decolorised by treating it for one or two or more minutes with a 

 mixture of sweet spirits of nitre and methylen-blue, malachite-green, 

 Bismarck brown, and gentian-violet. The preparation is then washed 

 with water. For sections the time required is longer (5 minutes). 

 They are then washed, nearly dried, immersed in carbol-xylol for 2 or 

 3 minutes, and then mounted in balsam. 



The smegma bacillus is decolorised by this method. 



Microscopical Injections with Albumen-Ink.f — Dr. O. Grosser re- 

 commends a method which appears to be very suitable for injecting 

 freshly killed small animals. White of egg is beaten up and after- 

 wards filtered through dry filter-paper. A stick of indian ink is then 

 rubbed up with the filtrate on a stone or on ground glass. Only a few 

 drops of the fluid are used at a time. The colour should be dark grey 

 and the consistence such that it will pass easily through the canula. 

 The advantages of this medium are that the albumen is coagulated by 

 the fixatives used, and therefore does not drop out or swell up. The 

 best fixatives are Miiller's fluid or picric acid in conjunction with 

 formalin. 



Fig. 204. 



Glass Staining Troughs.} — Prof. P. Schiefferdecker, after pointing 

 out the defects of stoneware staining troughs, states that glass vessels 



* Journ. Applied Microscopy, iii. (1900) pp. 898-900. 

 t Zeitschr. f. wiss. Mikr., xvii. (1900) pp. 178-81 (1 pi.). 

 j Tom. cit., pp. 167-8 (1 fi.«.). 



