Chapter XV: Bacteria 



111 



PATHOGENIC BACTERIA STAINED 

 BY GRAM'S METHOD 

 Bacillus aerogenes capsulatus 

 Bacillus of anthrax 

 Bacillus diphtheriae 

 Bacillus of malignant edema 

 Bacillus of tetanus 

 Bacillus of tuberculosis 

 Micrococcus tetragenus 

 Pneumococcus 



Staphylococcus pyogenes aureus 

 Staphylococcus pyogenes albus 

 Streptococcus pyogenes 



Streptococcus capsulatus 



PATHOGENIC BACTERIA DECOLORIZED 

 BY GRAM'S METHOD 



Bacillus of bubonic plague 

 Bacillus of chancroid 

 Bacillus coli communis 

 Bacillus of dysentery 

 Bacillus of glanders 

 Bacillus of influenza 

 Bacillus mucosus capsulatus 

 Bacillus proteus 

 Bacillus pyocyaneus 

 Bacillus of typhoid 

 Diplococcus intra cellularis 



meningitidis 

 Gonococcus 

 Spirillum of Asiatic cholera 



4. Staining Spores (Abbott's method). Prepare a cover-glass smear in 

 the usual way. Apply the stain (e. g., methylen blue) and hold the 

 cover-glass over a flame until the liquid steams. Repeat the heating 

 several times, but do not boil continuously. Rinse the cover-glass in 

 water and then decolorize the preparation in a 0.3 per cent, solution of 

 hydrochloric acid in 95 per cent, alcohol, until all color visible to the 

 naked eye has disappeared. Wash in water. If a counterstain is de- 

 sired, stain for from 8 to 10 seconds in anilin-fuchsin solution. Rinse 

 in water and mount in the usual way. The spores are stained blue. 



5. Staining Flagella (Bunge's modification of Loeffler's method). The 

 locomotor organs of motile bacteria are long, hairlike prolongations (1 to 

 many) termed flagella. Special methods of staining are necessary for 

 their demonstration. 



Make thin cover-glass smears of an 18-hour culture which contains 

 motile forms. Dry and fix in the ordinary way. 



The mordant. 



Ferric chloride, aqueous solution (1: 20) .... 25 c.c. 



Alum, saturated aqueous solution , 75 c.c. 



Shake well and add, 



Fuchsin (basic), saturated aqueous solution ... 10 c.c. 



Filter and allow to stand for some time before using. Treat the 

 smear for 5 minutes with this preparation, gently warming by holding 

 it high above a flame. The fluid must not boil. Rinse in water, then 

 stain faintly with carbol-fuchsin. Repeat the process until a successful 

 result is obtained. Mount in the usual way. 



