CONTENTS 



PAGE 



INTRODUCTORY . 1 



Apparatus and Supplies Required, 1; General Rules, 5. 



CHAPTER I. PREPARATION OF REAGENTS . 7 



Practical Exercise, 7; Memoranda, 13. 



.CHAPTER II. GENERAL STATEMENT OP METHODS ....... 15 



Killing, Fixing and Hardening, 16; Washing, 17; Dehydrating, 18; 

 Preserving, 18; Staining, 19; Clearing, 21; Mounting, 22; Imbedding, 

 22; Affixing Sections, 23; Decolorizing, 24; Bleaching, 24; Corrosion, 

 24; Decalcification and Desilicidation, 24; Injection 25; Isolation of 

 Histological Elements, 25; Normal or Indifferent Fluids, 25; Gen- 

 eral Scheme for Mounting Whole Objects (In Toto Preparations) or 

 Sections, 26. 



CHAPTER III. KILLING AND FIXING 27 



Cautions, 27; Alcohol Fixation, 28; Fixing with Gilson's Mercuro- 

 Nitric Mixture. 28; Fixing with Erlicki's Fluid, 29; Formalin as a 

 Fixing Reagent, 29; Memoranda. 30. 



CHAPTER IV. SIMPLE SECTION METHODS 33 



Free-Hand Section Cutting, 33; Memoranda, 34, 



CHAPTER V. THE PARAFFIN METHOD; IMBEDDING AND SECTIONING . 37 

 The Method, 37; Memoranda, 43; Tabular Statement of Difficulties 

 Likely to Be Encountered in Sectioning in Paraffin and the Probable 

 Remedy, 46. 



CHAPTER VI. THE PARAFFIN METHOD: STAINING AND MOUNTING . , 49 

 Staining with Hematoxylin, 49; Double Staining in Hematoxylin 

 and Eosin, 51; Double Staining in Carmine and Lyons Blue, 51; 

 Staining with Heidenhain's Iron-Alum Hematoxylin, 52; Bordeaux 

 Red and Iron-Hematoxylin, 53; Staining in Bulk before Section- 

 ing, 54; Paraffin Method for Delicate Objects, 54; Memoranda, 55. 



CHAPTER VII. THE CELLOIDIN METHOD 59 



The Method, 59; Staining Celloidin Sections in Hematoxylin and 

 Eosin, 62; Memoranda, 62. 



CHAPTER VIII. THE FREEZING METHOD . ....... 67 



The Method, 67; Memoranda, 69. 



CHAPTER IX. METALLIC SUBSTANCES FOR COLOR DIFFERENTIATION . 71 

 A Golgi Method for Nerve Cells and Their Ramifications, 71; Memo- 

 randa, 72; Silver Nitrate Method for Nerve, 73; Gold Chloride 

 Method for Nerve Endings, 74. 



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