viii Animal Microloyy 



CHAPTER X. ISOLATION OF HISTOLOGICAL ELEMENTS. MINUTE DIS- 

 SECTIONS 75 



Dissociation by Means of Formaldehyde, 75; Isolation of Muscle 

 Fibers by Maceration and Teasing, 75; Maceration by Means of 

 Hertwig's Fluid, 76; Minute Dissection and Mounting of Various 

 Parts of Insects, 77; Memoranda, 78. 



CHAPTER XI. TOOTH, BONE, AND OTHER HARD OBJECTS 79 



Sectioning Decalcified Tooth, 79; Sectioning Decalcified Bone, 79; 

 Sectioning Bone by Grinding, 80; Memoranda, 80. 



CHAPTER XII. INJECTION OF BLOOD AND LYMPH VESSELS .... 81 

 Red Injection Mass, 81; Blue Injection Mass, 81; Injection with a 

 Syringe, 81; Memoranda, 84. 



CHAPTER XIII. OBJECTS OF GENERAL INTEREST: CELL-MAKING, 



FLUID MOUNTS, "!N TOTO" PREPARATIONS, ETC 87 



Turning Cells, 87; Mounting in Glycerin (Water Mites, Transparent 

 Larvae), 88; Killing and Mounting Hydra, 89; Mounting in Glycerin 

 Jelly (Small Crustacea, etc.), 89; Mounting in Balsam (Flat Worms, 

 Mosquito, Gnat, Aphid), 90; Opaque Mounts (Beetles, Wings of 

 Moths and Butterflies, Head of Fly, Foreleg of Dytiscus), 92; 

 Memoranda (Including Directions for Mounting Other Forms), 93. 



CHAPTER XIV. BLOOD , 97 



Examination of Fresh Blood, 97; Effects of Reagents, 97; To Demon- 

 strate Blood Platelets, 97; Stained Preparation of Fibrin, 97; Crystals 

 of Blood, 97; Cover-Glass Preparations (Dry), 98; Rapid Method, 99; 

 Enumeration of Blood Corpuscles, 99; Observation of the Blood 

 Current, 101; Inflammation, 101; Memoranda, 101. 



CHAPTER XV. BACTERIA 105 



Bacterial Examination, 105; Cover-Glass Preparation from Fluid 

 Media, 105; Staining and Mounting, 106; Bacteria in Tissues, 107; 

 Methylen Blue Stain for Bacteria in Tissues, 107; Gram's Method for 

 Bacteria in Tissues, 107; Hanging-Drop Preparations, 108; Memo- 

 randa, 108. 



CHAPTER XVI. SOME EMBRYOLOGICAL METHODS: THE CHICK; SEC- 

 TIONS AND WHOLE MOUNTS; OTHER FORMS 113 



The Chick, 113; Series of Chick Embryos for a Course in Embry- 

 ology, 115; Measuring the Length of Embryos, 116; Orientation of 

 Young Chick Embryos, 116; Preparation of Material for the Embry- 

 ology of Teleosts, 117; Preparation of Material for the Embryology 

 of Amphibia, 118; Early Stages of the Mammalian Embryo, 119; 

 Older Stages of the Mammalian Embryo, 120; Maturation, Fertiliza- 

 tion, and Segmentation in Mammals, 121; Artificial Fecundation 

 (Echinoderms, Amphibia, Teleosts), 122; Early Cleavage in Living 

 Material, 123; Polar Bodies, Fertilization and Early Cleavage in 



