Chapter I: Preparation of Reagents 9 



Caution. In handling 1 , corrosive sublimate do not use metal 

 instruments because it corrodes metal. Use a glass or horn 

 spatula. 



8. Erlicki's Solution. 



Bichromate of potash 5 grams 



Sulphate of copper 2 grams 



Distilled water 220 c.c. 



Pulverize the crystals before adding the water. 



9. Gage's Carbol-Xylol Clearer. 



Carbolic acid crystals (melted) 25 parts 



Xylol 75 parts 



If the carbolic acid does not dissolve in the xylol, increase the 



amount of the latter. Handle the acid with great care. Quantity, 



250 c.c. 



10. Gage's Formaldehyde Dissociator. 



Formalin, commercial 0.5 c.c. 



Normal saline 250.0 c.c. 



11. Decalcifying Solution. 



Nitric acid (strong) 10 c.c. 



Alcohol (70 per cent.) 90 c.c. 



12. Borax Carmine Stain (Grenadier's). 



Borax (4 per cent, aqueous solution) .... 100 c.c. 

 Carmine 1 gram 



Heat until the carmine dissolves, then add 100 c.c. of 70 per 

 cent, alcohol. Filter after 24 hours. 



13. Delafield's Hematoxylin. Prepare 100 c.c. of a saturated 

 aqueous solution of ammonia alum. Dissolve 1 gram of hema- 

 toxylin crystals in 10 c.c. of absolute alcohol, and add it, drop 

 by drop, to the first solution. Expose this mixture to air and 

 light for several weeks (two months is not too long) to "ripen." 

 (Ripening consists in an oxidation of the hematoxylin to form 

 hematein. This may be accomplished at once with some degree 

 of success through the addition of a few cubic centimeters of a 

 neutralized solution of peroxide of hydrogen.) When ripe, filter 

 the solution and add 25 c.c. of glycerin, and 25 c.c. of methyl 

 alcohol (see memorandum 1). It is well to have a stock solution 



