12 



Animal Micrology 



21. Mayer's Albumen Fixative. -Chop the white of an egg 

 with scissors and filter it through moist filter paper. It filters 



through very slowly. Add an 

 equal volume of glycerin, and a 

 bit of salycilate of soda (1 gram 

 to 50 c.c.) or thymol to prevent 

 putrefaction. 



22. Celloidin. Put 5 grams of 

 Sobering' s shredded or granular 

 celloidin into a celloidin bottle (a 

 bottle with glass stopper and 

 ground glass cap) and dissolve it 

 in equal parts of absolute alcohol 

 and ether (see 4). Add only suf- 

 ficient fluid (about 100 c.c.) to 

 make a thick, syrupy mass. In a 

 second celloidin bottle make a thin 

 solution by taking about one-third 



FIG. 25. Simple Water-Bath. 



This is a useful bath for individual 

 workers. It is provided with imbedding- 

 cups, infiltration vials, a shelf for watch- 

 glass imbedding or for warming instru- 

 ments, and tubulatures for gas regulator 

 and thermometer. 



of the original solution and dilut- 



Label the 



ing it with its own volume of the ether-alcohol, 

 bottles thick and thin celloidin, respectively. 



23. Paraffin. In one of the cups of a warm paraffin oven 

 (Fig. 24, 25, or 26), put 75 grams of paraffin, melting at about 

 53 C. The bath should be kept 

 at a temperature of some two 

 degrees above the melting-point 

 of the paraffin. A supply of softer 

 and of harder paraffin (e. g., melt- 

 ing at 43 and 60 C.) should also 

 be at hand. 



Other Reagents. Provide your- 

 self with 200 c.c. of xylol, 25 c.c. 

 of clove oil, 25 c.c. of glacial acetic 

 acid, 50 c.c. of cedar-wood oil, 75 

 c.c. of chloroform, 30 c.c. of gly- 

 cerin and 250 c.c. of absolute 



FIG. 26. Imbedding-Table. 



There should be two rectangular 

 boxes (about 3X3X16 cm.) to contain 

 paraffin. When in use the boxes are so 

 placed on the imbedding-table that the 

 paraffin in one end remains melted ; in 

 the other, solid. Regulate the tempera- 

 ture by placing the flame at the proper 

 distance under the acute angle of the 

 table. It is best, when gas is used, al- 

 ways to turn on the gas completely and 

 then regulate the height of the flame by 

 means of a clamp on the rubber tubing 

 which conducts gas to the burner. 



