26 



Animal Micrology 



GENERAL SCHEME FOR MOUNTING WHOLE OBJECTS ( IN TOTO PREP- 

 ARATIONS) OR SECTIONS 



Whole Objects (for balsam mounts) Section Methods (paraffin and celloidin) 

 Killing and fixing Killing and fixing 



Washing 

 Staining 



(Decolorizing if necessary) 



I 

 Dehydrating 



Clearing 

 Mounting 



Washing 



(Staining, if to be stained in bulk) 

 Hardening and dehydrating 

 Absolute alcohol 



Paraffin Method 

 Dealcoholization (xylol) 



If not stained 

 in bulk 



Through alco- 

 hols to stain 



I 

 Staining 



Washing 



I 



Dehydrating 

 ( and decol- 



orizing if 

 necessary) 



Melted paraffin 



Imbedding 



Sectioning 



Affixing sections 



I 

 Removal of paraffin 



I 

 Absolute alcohol 



Clearing 



// not stained in 



bulk 

 Staining 



I 



Washing (and de- 

 colorizing if 

 necessary) 



Celloidin Method 

 Ether-alcohol 



Thin celloidin 



Thick celloidin 



I 

 Imbedding 



I . 

 Sectioning* 



I 



Dehydrating to 

 95 per cent, al- 

 cohol 



I 

 Clearing 



I 

 Mounting 



Mounting 

 * If sections are to be arranged serially they must be affixed to the slide as soon as cut. 



