222 Animal Micrology 



Carney's fluid (reagent 2) before dehydrating or staining. If 

 desired, the tissue may be sectioned in celloidin or paraffin. 



To Demonstrate Living Trichinae Barnes (American Monthly 

 Microscopical Journal, Vol. XIV, p. 104) subjects small bits of 

 trichinized muscle to a mixture of 3 grains of pepsin, 2 drams of 

 water, and 2 minims of hydrochloric acid, for about three hours at 

 body temperature with occasional shaking. When the flesh and 

 cysts are dissolved, the liquid is poured into a narrow glass 

 vessel and allowed to settle. The live trichinae may be withdrawn 

 with a pipette from the bottom of the fluid and examined on a 

 warm stage. 



ROTIFERS 



Rotifers will usually be found in abundance in some of the 

 laboratory aquaria on the lighted side of the vessel. For ordinary 

 class work they are best studied alive. They are difficult to 

 preserve properly. Full directions for killing and preserving 

 will be found in Jenning's paper, "Rotatoria of the United States," 

 U. S. Fish Commission Bulletin, 1902, p. 277. 



To Quiet Rotifers, Cole (Journal of Applied Microscopy, Vol. 

 VI, p. 2179) anaesthetizes them by adding from time to time a 

 drop of 1 per cent, aqueous solution of chloretone to the water on 

 the slide in which the animals are being examined. 



BRYOZOA 



They may be treated in the same way as compound hydrozoa. 

 Plumatella may frequently be found in shallow fresh-water 

 streams on the under side of flat rocks; Pectinatella, in rivers 

 and streams on the upper surface of mussel shells, etc. 



EARTHWORM 



Earthworms are best collected on warm, rainy nights when 

 they may be found extended on the surface of the ground near 

 their burrows. They are most plentiful in. old gardens or rich 

 lawns. A lantern and a pail are the only implements necessary. 

 Earthworms may frequently be found, however, in large numbers 

 on the surface of the ground on cloudy days immediately after 

 prolonged hard rain. 



