70 DKOSEEA ROTUNDIFOLIA. CHAP. l\ 



distinctly seen in a small mass of protoplasm in one of the cells 

 of a tentacle.* After a few hours all the tentacles and thf 

 blade became inflected. 



Experiment 2. Another leaf was plunged into water at 130'" 

 to 131, and as before there was no inflection. After being kepf 

 in cold water for an hour, it was placed in the strong solutior 

 of ammonia, and in the course of 55 m. the tentacles were con- 

 siderably inflected. The glands, which before had been rendered 

 of a brighter red, were now blackened. The protoplasm in the 

 cells of the tentacles was distinctly aggregated ; but the spheres 

 were much smaller than those usually generated in unheated 

 leaves when subjected to carbonate of ammonia. After an 

 additional 2 hrs. all the tentacles, excepting six or seven, were 

 closely inflected. 



Experiment 3. A similar experiment to the last, with exactly 

 the same results. 



Experiment 4. A fine leaf was placed in water at 100 (37 '7 

 Cent.), which was then raised to 145 (62 '7 Cent.). Soon after 

 immersion, there was, as might have been expected,, strong 

 inflection. The leaf was now removed and left in cold water ; 

 but from having been exposed to so high a temperature, it 

 never re-expanded. 



Experiment 5. Leaf immersed at 130 (54'4 Cent.), and the 

 water raised to 145 (62 '7 Cent.), there was no immediate in- 

 flection ; it was then placed in cold water, and after 1 hr. 20 m. 

 some of the tentacles on one side became inflected. This 

 leaf was now placed in the strong solution, and in 40 m. all 

 the submarginal tentacles were well inflected, and the glands 

 blackened. After an additional interval of 2 hrs. 45 m. all the 

 tentacles, except eight or ten, were closely inflected, with their 

 cells exhibiting a slight degree of aggregation ; but the spheres 

 of protoplasm were very small, and the cells of the exterior 

 tentacles contained some pulpy or disintegrated brownish 

 matter. 



Experiments 6 and 7. Two leaves were plunged in water at 

 135 (57 ' 2 Cent.) which was raised to 145 (62 7 Cent.) ; neither 

 became inflected. One of these, however, after having been left 

 for 31 m. in cold water, exhibited some slight inflection, which 

 increased after an additional interval of 1 hr. 45 m., until 



* Sachs states (' Traite de Bo- after they were exposed for 1 m. 



tanique,' 174, p. 855) that the iu water to a temperature of 47 



movements of the protoplasm in to 48 Cent., or 1 17 to 1 19 



the hairs cf a Cucurbita ceased Fahr. 



