UTRICULARIA NEGLECTA. OHAP. XVn. 



change perceptible in the quadrifids or bifids after 21 hrs. 

 Four bladders were then treated- in the same manner with a 

 solution of one part of nitrate of ammonia to 437 of water, and 

 re-examined after 21 hrs. In two of these the quadrificls now 

 appeared full of very finely granular matter, and their proto- 

 plasmic lining or primordial utricle was a little shrunk. In the 

 third bladder, the quadrifids included distinctly visible granules, 

 and the primordial utricle was a little shrunk after only 8 hrs.' 

 In the fourth bladder the primordial utricle in most of the 

 processes was here and there thickened into little, irregular, 

 yellowish specks; and from the gradations which could be 

 traced in this and other cases, these specks appear to give rise 

 to the larger free granules contained within some of the pro- 

 cesses. Other bladders, which, as far as could be judged, had 

 never caught any prey, were punctured and left in the same 

 solution for 17 hrs. ; and their quadrifids now contained very 

 fine granular matter. 



A bladder was bisected, examined, and irrigated with a 

 solution of one part of carbonate of ammonia to 437 of water. 

 After 8 hrs. 30 m. the quadrifids contained a good many granules, 

 and the primordial utricle was somewhat shrunk ; after 23 hrs. 

 the quadrifids and bifids contained many spheres of hyaline 

 matter, and in one arm twenty-four such spheres of moderate 

 size were counted. Two bisected bladders, which had been 

 previously left for 21 hrs. in the solution of gum (one part to 

 218 of water) without being affected, were irrigated with the 

 solution of carbonate of ammonia ; and both had their quadrifids 

 modified in nearly the same manner as just described, one 

 after only 9 hrs., and the other after 24 hrs. Two bladders 

 which appeared never to have caught any prey were punctured 

 and placed in the solution ; the quadrifids of one were examined 

 after 17 hrs., and found slightly opaque; the quadrificls of the 

 other, examined after 45 hrs., had their primordial utricles more 

 or less shrunk with thickened yellowish specks, like those due 

 to the action of nitrate of ammonia. Several uninjured bladders 

 were left in the same solution, as well as in a weaker solution 

 of one part to 1750 of water, or 1 gr. to 4 oz. ; and after two 

 days the quadrifids were more or less opaque, with their con- 

 tents finely granular ; but whether the solution had entered by 

 the orifice, or had been absorbed from the outside, I know not. 



Two bisected bladders were irrigated with a solution of one 

 part of urea to 218 of water ; but when this solution was em- 

 ployed, I forgot that it had been kept for some clays in a warm 

 room, and had therefore probably generated ammonia ; anyhow 



