420 UTRICULARIA NEGLECTA. CHAP. XVII. 



meat. No change in the glands was observable for some hours, 

 but after 24 hrs. most of thorn had become brownish, and more 

 opaque and granular than they were before. In these speci- 

 mens, as in those irrigated with the salts of ammonia, the 

 nuclei seemed to have increased both in size and solidity, but 

 they were not measured. Five summits were also irrigated 

 with a fresh infusion of raw meat; three of these were not at 

 all affected in 24 hrs., but the glands of the other two had 

 perhaps become more granular. One of the specimens which 

 \v;is not affected was then irrigated with the mixed solution of 

 the nitrate and phosphate of ammonia, and after only 25 m. 

 the glands contained from four or five to a dozen granules. 

 After six additional hours their primordial utricles were greatly 

 shrunk. 



The summit of a bladder was examined, and all the glands 

 found colourless, witli their primordial utricles not at all 

 shrunk; yet many of the oblong glands contained granules just 

 resolvable with No. 8 of Hartnack. It was then irrigated with 

 a few drops of a solution of one part of urea to 218 of water. 

 After 2 hrs. 25 m. the spherical glands were still colourless; 

 whilst the oblong and two-armed ones were of a brownish tint, 

 ;unl their primordial utricles much shrunk, some containing 

 distinctly visible granules. After 9 hrs. some of the spherical 

 glands were brownish, and the oblong glands were still more 

 changed, but they contained fewer separate granules; their 

 nuclei, on the other hand, appeared larger, as if they had 

 absorbed the granules. After 23 hrs. all the glands were 

 brown, their primordial utricles greatly shrunk, and in many 

 cases ruptured. 



A bladder was now experimented on, which was already 

 somewhat affected by the surrounding water ; for the spherical 

 glands, though colourless, had their primordial utricles slightly 

 shrunk; and the oblong glands were brownish, with their 

 utricles much, but irregularly, shrunk. Ihe summit was 

 treated with the solution of urea, but was little affected by it in 

 U hrs. ; nevertheless, after 23 hrs. the spherical glands were 

 brown, with their utricles more shrunk ; several of the other 

 glands were still browner, with their utricles contracted into 

 irregular little masses. 



Two other summits, with their glands colourless and their 

 utricles not shrunk, were treated with the same solution of 

 urea. After 5 hrs. many of the glands presented a shade of 

 brown, with their utricles slightly shrunk. After 20 hrs. 

 40 m. -some few of them were quite brown, and contained 



