PROC. ENT. SOC. WASH., VOL. 22, NO. 6, JUNE, IQ2O 141 



of one form appeared to be darker the latter possibly due to 

 unfavorable moisture conditions. Very few workers and no sol- 

 diers were observed; the colony appeared to be moribund. 



A more careful examination on October 4, 1919, revealed that 

 the darkly pigmented form was a male of the normal second form 

 with short wing pads, whereas the lighter colored form was a 

 female with very short or vestigial wing pads an intermediate. 

 The abdomen of this queen was not distended. Only (i living 

 workers were found. The colony was still alive and was watched 

 with considerable interest to observe developments. It was not 

 believed that these old forms could thrive or reproduce without 

 a large number of workers to care for and feed them. 



On December 15, 1919, all the termites in this colony were 

 dead. 



The criticism can scarcely be made that there has not been 

 sufficient time for the production of winged forms, for even in 

 recently established incipient colonies in nature the nymphs of 

 the winged reproductive forms are produced after IS months. 



Heath (1903) found winged adult termites of Termopsis angus- 

 ticollis Hagen swarming from nests in which males and females 

 of the first form were present, the nests being only two years old 

 and containing 200 individuals. 



The writer has made observations on the habits of termites 

 since 1912, mainly in the southeastern United States, but in the 

 season of 1917, during an extensive field trip through Florida, 

 the southwest, the Rocky Mountain and the Pacific Coast regions. 

 It may be stated with certainty that: (1) In long-established 

 colonies, in which large fertilized queens of the second form oc- 

 curred, no nymphs or winged adults of the first form have been 

 found; (2) in all colonies in which queens of the third form were 

 found, no nymphs or winged adults of the first form occurred; 

 most of these colonies, however, were small or young, that is, 

 they had been recently established. 



In one large colony of R. ftavipes in Virginia, with 17 third 

 form queens present, a few nymphs of the second form were 

 found. 



In species of Reticulitermes, mature second form queens, like 

 those of the first form, will not survive in artificial colonies unless 

 there are a large number of workers present to care for them. 



No mature second form adults have as yet been found in col- 

 onies of Prorhinotermes simplex. 



Insufficient specimens of reproductive individuals of the second 

 form have been thus far found in colonies of Termopsis and Kalo- 

 termes to warrant the drawing of definite conclusions. 



In the higher termites the Termitinae Banks such as Ami- 



