84 bulletin: museum of comparative zoology. 



for example by Davis ('08) for Dissosteira and Stenobothrus, and 

 McClung ('14) for various Acrididae. But no one, so far as I am 

 aware, has described such a condition for any of the autosomes. 

 Figure 99 is from a sUde that had been treated with Heidenhain's 

 iron-haematoxyhn stain, but the destaining process had been carried 

 farther than in most of the other sUdes. Figure 100 is from another 

 individual, the slides of which had been stained by Flemming's tri- 

 color method, but had not been excessively differentiated. It will be 

 noted that the autosomes in this figure do not exhibit the roughened 

 synaptic ends that are seen in figure 99. It seems probable, therefore, 

 that differences in the staining process may have much to do with 

 the appearance or non-appearance of the roughened condition. In 

 heavily stained slides even the accessory, as well as the tetrads B and 

 C, may appear with a smooth contour. In this connection, I may call 

 attention to these several points: — (1) Tetrad B is unequal in both 

 the cases figured and the roughened end corresponds to the large 

 distal granule on the larger conjugant (see fig. 64, Plate 6). (2) 

 Tetrad C is likewise unequal and the roughened end also corresponds 

 with the large distal granule at the end of the larger of the two com- 

 ponents (see fig. 65). (3) The polar granules usually occur at the 

 proximal end, i. e. the end to which the spindle-fibers attach, and 

 therefore the roughened tips of the autosomes in figure 99 probably 

 correspond to the polar granules of these elements. (4) The accessory 

 chromosome and the polar granules have the common property of 

 remaining condensed while the rest of the chromatin is diffuse, as well 

 as the common property exliibited in these two figures (99 and 100, 

 Plate 9). The suggestion therefore offers itself that there may be 

 some common physical or chemical properties underlying the corre- 

 spondence in behavior between the accessory and the polar granules. 



3. Chromosovw-pair C. — The drawings of chromosome-pair B in 

 figure 64 (Plate 6) and those of C in figure 65 were made from sections 

 cut from the same testis. An examination of the spermatogonial 

 telophases of tliis individual revealed the larger members of each of 

 these pairs very well defined, as indicated in figures 101-105 (Plate 9). 

 No attempt was made to recognize the smaller members of these pairs, 

 because they lacked characteristics, other than size, distinctive enough 

 to make recognition certain. With the larger members of these pairs, 

 however, the distinguishing features are so pronounced that I think 

 there can be no doubt about the identification. 



I did not attempt to follow these elements through the preleptotene 

 and leptotene stages, but I have no doubt that careful enough study 



