538 



ECOLOGY AND BEHAVIOR 



Major 

 Hbs 



Minor Hb 



Non-haem 

 Protein 



*£» 



aa 



cc 



origin 



ac 



bb 



bb 



Figure 5 Hemolysates from individual sharks were separated on cellulose acetate gels 

 by ionophoresis at pH 8.6. The polymorphism of the non-haem protein was detected 

 by staining the gel with 0.2% Amido Black. On the gel shown there are four of the six 

 possible phenotypes. 



Table 3. Gene frequencies, observed and expected phenotypes, chi-square and 

 probabilities for haemolysates for various populations of H. portusjacksoni. 



Phenotypes 



Locality 



O/E aa ab ac bb be cc 



Gene frequencies 



Newcastle Obs. 2 



Sydney 

 Jervis Bay 

 Victoria 



24 



Exp. 6.5 15.5 5 



Obs. 2 13 11 



Exp. 1.7 15.6 9 



Obs. 6 14 17 



Exp. 4.6 16.7 17 



Obs. 2 



Exp. 0.5 2.6 



S. Australia Obs. 6 4 



Exp. 4 7.3 



W. Australia Obs. 3 3 



Exp. 2.7 6 



9.5 6.1 1 



36 42 10 



35.3 40.6 11.7 



16 31 15 



14.9 30.3 15.4 

 4 5 4 3 



2.3 3.5 6.2 2.7 

 6 5 6 3 



6.6 3.3 6 2.7 



13 6 8 12 



11 2.9 11.5 11.2 



0.386 0.466 0.148 9.0 <0.05 



0.123 0.557 0.320 1.23 <0.8 



0.217 0.388 0.394 0.97 <0.9 



0.166 0.444 0.389 3.3 <0.5 



0.366 0.333 0.300 3.4 <0.5 



0.244 0.255 0.500 6.3 <0.1 



so difficult to obtain adequate numbers of animals for all but two of the 

 populations. It must also be considered that only the Sydney and Jervis 

 Bay populations were sampled inshore and on the breeding grounds; hence, 

 it is possible that the animals taken in the other areas are not all members 

 of the same subpopulation. However, in general the results illustrate well 

 some of the basic principles of population genetics. They also lend some 

 support to the theory of the presence of reproductively semi-isolated 



