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ECOLOGY AND BEHAVIOR 



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Figure 3 Alterations in the histological appearance of the interrenal glands of captive 

 Squalus acanthias. (a) Interrenal of a freshly caught animal, stained for lipid contents 

 (formalin fixed, frozen section, 10 /urn); Sudan Black B, X40. (b) Interrenal of an animal 

 sacrificed after 18 days in captivity, stained for lipid contents (processed as in 2a); Sudan 

 Black B, X40. (c) Interrenal of a freshly caught animal, stained for lipid contents 

 (processed as in 3a); Sudan Black B. X 250. (d) Interrenal of an animal sacrificed after 18 

 days in captivity (processed as in 3a); Sudan Black B, X 250. (e) Parenchymal cells of a 

 freshly caught animal (formalin fixed, glycol-methacrylate embedding, 4 jum); 

 hematoxylin and eosin, X 500. (f ) Parenchymal cells of an animal sacrificed after 9 days in 

 captivity, showing cells with low lipid content and homogeneous cytoplasm (processed as 

 in 3a); hematoxylin and eosin, X 500. 



