FASTING CONFINEMENT EFFECTS ON SHARKS 



619 



Table 3. Alterations in nuclear diameter in cells of the 

 interrenal gland. 



Animal 



Mean diameter 

 of nuclei 

 (n = 50) 



Result of 

 Tukey test 

 at 0.01 

 level* 



Wild control 

 Sacrificed active 

 Sacrificed immobile 

 Died in pen 



8.6m ± 0.7f 



9.7 ±0.7 

 8.5 ±1.2 



7.8 ±0.5 



Significant 

 Significant 

 Not significant 



*As compared with the preceding mean diameter. 

 tMean ± standard deviation. 



Table 4. Gland cholesterol, modified Schultz reaction. 



Animals 



Color index 



Wild population controls 



Sacrificed after 6-14 days in captivity— actively 

 swimming 



Sacrificed when immobile 



Samples collected after death in pen 



19 

 17 

 20 

 19 

 19 

 21 

 11 

 10 

 3 

 



reticuloendothelial cells of the sheathed arteries. This pigment did not stain 

 for iron with any of the tests used (Gomori's, Hutchinson's, Turnbull Blue, 

 or bathophenanthroline techniques, following buffered formalin fixation). 

 Hemmeter (1926) described a pigment of similar appearance in the arterial 

 sheaths in the spleen of Alopias, and attributed it to products of erythrocyte 

 destruction. This pigment has the histochemical characteristics of hematoi- 

 din. Figure 5a-f summarizes the alterations observed in the spleens of ex- 

 perimental animals. 



Little agreement exists in the literature concerning the origins and iden- 

 tities of other hematological elements of elasmobranchs. The following cell 

 types, which will be described in detail elsewhere (Martini, in preparation) 

 will be referred to in this paper: lymphocytes, monocytes, thrombocytes, 

 polymorphs, eosinophils I (fine, rod-shaped granules found in the circulatory 



