FASTING CONFINEMENT EFFECTS ON SHARKS 



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Figure 5 (a) Normal spleen, from a freshly caught animal (formalin fixed, paraffin 

 embedding, 6 Mm); hematoxylin and eosin, X 40. (b) Spleen from an animal sacrificed 

 after 13 days in captivity (processed as in 5a); hematoxylin and eosin, X 40. (c) Spleen 

 from an animal that died in the pen after 18 days in captivity (processed as in 5a); 

 hematoxylin and eosin, X 40. (d) Spleen from an animal that died in the pen after 22 days 

 in captivity (processed as in 5a); hematoxylin and eosin, X40. (e) Reticuloendothelial 

 cells of the sheathed arteries, normal spleen (formalin fixed, glycol-methacrylate 

 embedding, 4 jum); hematoxylin and eosin, X 500. (f) Cells of the sheathed arteries from 

 an animal that died in the pen after 22 days of captivity (processed as in 5a); hema- 

 toxylin and eosin, X 500. 



