Collections were made by crews operating out of 

 Montclair State College and the New Jersey Marine 

 Sciences Consortium Seaville Field Station; Gulf 

 Coast Research Laboratory, Ocean Springs, Miss.; 

 Texas A&M University of Galveston Marine Labo- 

 ratory at Galveston, Tex.; University of Southern 

 California Institute for Marine and Coastal Studies, 

 Los Angeles, Calif.; and the Southwest Fisheries 

 Center Tiburon Laboratory of the National Marine 

 Fisheries Service (NMFS), Tiburon, Calif. Sampling 

 of target species was accomplished by using appro- 

 priate gear, including beach seines, gill nets, otter 

 trawls, and hook and line. Following capture, 

 specimens were cooled and stored in ice. Subsequent- 

 ly, all specimens were measured, weighed, sexed, 

 and aged. Fish were then filleted, the right side serv- 

 ing for analysis samples and the left for NMFS 

 archives. Gonad and liver tissues also were archived 

 for future reference. All samples were frozen in pre- 

 rinsed aluminum foil prior to shipment to Gloucester. 

 All samples were composited at the Gloucester 

 Laboratory and consisted of equal weights of 10 

 deboned, skinless fillets from the right side of 10 in- 

 dividual fish. Target species included are shown on 

 Table 1 and are arranged in phyletic sequence, ac- 

 cording to families to which they belong. 



Analytical procedure was in accordance with the 

 AOAC multiresidue procedure for pesticides (Hor- 



Table 1. — Common and scientific names of fish species 

 listed in phylogenetic order. 



Gulf menhaden 



Gafftopsail catfish 



Red hake 



Atlantic tomcod 



Whiting (silver hake) 



Striped bass 



White perch 



Kelp bass 



Bluefish 



Sheepshead 



Weakfish 



Speckled trout 



Spot 



Black drum 



Red drum 



Silver perch 



Atlantic croaker 



Southern kingfish 



White croaker 



Opaleye 



Halfmoon 



White seaperch 



Striped mullet 



Chub mackerel 



Spanish mackerel 



California scorpionfish 



Winter flounder 



Summer flounder 



Windowpane flounder 



Hogchoker 



Pacific sanddab 



Southern flounder 



Brevoortta patronus 

 Bagre mannus 

 Urophycis chuss 

 Microgadus tomcod 

 Merluccius bi linearis 

 Morone saxatilis 

 Morone amencana 

 Paralabrax clathratus 

 Pomatomus saltatrix 

 Archosargus probatocephalus 

 Cynoscion regalis 

 Cynoscton nebulosus 

 Leiostomus xanthurus 

 Pogomas cromts 

 Sc/aenops ocellatus 

 Bairdtella chrysoura 

 Mtcropogon/as undulatus 

 Menticirrhus amencanus 

 Genyonemus /meatus 

 Girella nigricans 

 Medialuna californiensis 

 Phanerodon furcatus 

 Mugil cephalus 

 Scomber japonic us 

 Scomberomorus maculatus 

 Scorpaena guttata 

 Pseudopleuronectes americanus 

 Paralichthys dentatus 

 Scophthalmus aquosus 

 Trinectes maculatus 

 Citharichthys sordidus 

 Paralichthys lethostigma 



witz 1980). Briefly, homogenates were extracted with 

 petroleum ether. The extract was concentrated, the 

 solvent completely removed, and the weight of fat 

 determined. Three grams or less of fat were taken for 

 acetonitrile partitioning between petroleum ether. 

 The extract was concentrated to ca. 10 ml and 

 transferred to a florisil column. PCB's were eluted 

 with 6% diethyl ether in petroleum ether, concen- 

 trated to 5 ml, and analyzed by gas-liquid chroma- 

 tography. The florisil extract was further 

 concentrated or diluted for eventual cleanup by 

 silicic acid chromatography (Armour and Burke 

 1970). A suitable aliquot was charged onto the 

 column. PCB's were eluted with petroleum ether, 

 concentrated, and made up to a definite volume. An 

 aliquot of the silicic acid extract was injected on a 

 Perkin-Elmer Sigma 1 gas chromatograph, equipped 

 with a Ni 63 electron capture detector. A 6-ft by 2 mm 

 i.d. glass coiled column consisting of 1.5% SP-2250 + 

 1.95 SP-2401 on 100/120 mesh Supelcoport was 

 used as the analytical column. The carrier gas was 

 argon/methane 95/5 at a flow rate of 20 ml/min. A 

 makeup flow of 40 ml/min was added for a total 

 detector flow of 60 ml/min. Injector temperature was 

 set at 225°C, detector 300°C, and oven 200°C. The 

 electrometer range was set at 1.0 nA. Efficiency of 

 the column for p, p'-DDT was determined to be 931 

 theoretical plates per foot. 



PCB's were measured by comparing total area of 

 residue peaks with total area of peaks from appro- 

 priate Aroclor reference material. Only those peaks 

 from samples that could be attributed to chloro- 

 biphenyls and which were present in the chroma- 

 togram of reference material were used. PCB 

 residues, with chromatographic patterns which were 

 altered extensively from Aroclor references, were 

 measured by individual peak area comparisons, us- 

 ing Aroclor reference material weight factors. Each 

 PCB peak was calculated against an appropriate in- 

 dividual reference peak with exactly the same ab- 

 solute retention time. Total PCB's were obtained by 

 summing individual peak values. 



Quality Assurance Program 



Before processing any samples, a method blank 

 (minus flesh) was run to insure that all glassware, 

 reagents, and solvents were interference free. Each 

 time there was a new set of samples, or occasionally 

 to check reagents, a method blank would be pro- 

 cessed as a safeguard against chronic contamination. 

 Standard quality assurance practices were used with 

 this method. For checking the accuracy of PCB 

 determinations, check standards were prepared. 



391 



